附子配伍原则的电喷雾质谱研究

目的从化学角度探讨附子配伍的原则。方法利用电喷雾质谱分析和比较生附子及其复方中药煎煮液中二萜类生物碱的变化规律；测定附子及其他中药煎煮液的pH值并用电喷雾质谱考察酸性对药材中乌头碱水解的影响。结果生附子单煎煮及与甘草共煎后双酯型乌头生物碱大部分发生水解，生附子与半夏或五味子共煎生物碱水解受抑制，生附子和甘草单煎液的酸性远低于半夏或五味子单煎液，中乌头碱在酸性条件下不水解。结论酸性是影响乌头碱类双酯型生物碱水解的重要因素，强酸条件下水解受抑制，附子煎煮液毒性增加。

Study on the combination principles about aconite roots by electrospray ionization mass spectrometry

Aim To explore the combination principle about aconite roots from the chemical point of view. Methods Analyzing and comparing the nordeterpenoid alkaloids in the crude aconite roots, the decoction of crude aconite roots and aconite roots with some other Chinese traditional herbs by electrospray ionization trap tandem mass spectrometry. Detecting the pH value of the decoction of crude aconite roots and of some other Chinese traditional herbs; studying the influence of acidity to the hydrolysis of standard sample of mesaconitine. Results Most of diester-diterpenoid alkaloids in the decoction of aconite roots and the decoction of aconite roots with liquorice roots are hydrolyzed, and the hydrolysis of alkaloids are inhibited in the decoction of aconite roots with Pinellia tuber or Schisandra. The acidity of the decoction of aconite roots or liquorice is lower than the decoction of Pinellia tuber or Schisandra; standard sample of mesaconitine can not be hydrolyzed in acid water. Conclusion Acidity is a key factor to influence the hydrolysis of diester-diterpenoid alkaloids, and the hydrolysis of alkaloids are inhibited in high acidity, which lead to the high toxicity of the decoction containing aconite roots.