比较脐血与成人外周血中T淋巴细胞亚群分布及其因子产生的差异

目的：探讨脐血与成人外周血中淋巴细胞亚群分布及细胞因子的差异及其与移物抗宿主病（GVHD）发生的关系，方法：采用流式细胞术，三色荧光单克隆抗体标记对脐血和成人外周血中淋巴细胞进行分类，并对其分泌的IL－2，TNF－α和IFN－r进行分析比较，结果：（1）脐血中T淋巴细胞以CD^4 ,CD45RA^ 细胞为主，而成人外周血以CD^ ,CD4R5RO^ 和CD^ 8CD450^ 两种表型为主，（2）脐血淋巴细胞产生IL－2，TNF－α和IFN－r的量明显低于成人外周血淋巴细胞，进一步分析表明，脐血中产生细胞因子的细胞大部分为CD^ 4CD45^ 细胞，而成人外周血中大部分为CD^ 4CD45RO^ 和CD^ 8CD^ 45RO细胞。结论：成人外周血与脐血淋巴细胞不仅存在亚群比例上的差异，而且亚群分泌细胞因子的量也明显减少，脐血干细胞移植的GVHD发生率较低可能与脐血中淋巴细胞亚群的比例不同及分泌细胞因子减少有关。

The difference of intracellular cytokines produced by different lymphocyte subsets of cord blood and adult peripheral blood

OBJECTIVE: To explore the difference of intracellular cytokines between cord blood and adult peripheral blood and elucidate its significance for GVHD. METHOD: Different lymphocyte subsets were categorized in cord and adult peripheral blood by monoclonal antibodies with 3 color fluorescence using flow cytometry. The IL-2, TNF-alpha and IFN-gamma produced by various lymphocyte subsets were compared between the two groups. RESULT: (1) In cord blood, dominant T cells were CD(4)(+)CD(45RA)(+), while the major T cells were both CD(4)(+)CD(45RO)(+) and CD(8)(+)CD(45RO)(+) in adult peripheral blood. (2) The quantities of IL-2, TNF-alpha and IFN-gamma produced by cord blood lymphocytes were lower than that of adult peripheral blood lymphocytes. Moreover, the majority of cells that produced cytokines in the cord blood were CD(4)(+)CD(45RA)(+), but in adult peripheral blood were mainly CD(4)(+)CD(45RO)(+) and CD(8)(+)CD(45RO)(+). CONCLUSION: As compared with adult peripheral blood, the cord blood not only possessed different proportion of lymphocyte subsets, but also significant low level of cytokines. The differences of various lymphocyte subset proportions and lower level of cytokines in cord blood probably related to the lower incidence of GVHD in cord blood stem cell transplantation.