高压氧治疗对严重烧伤大鼠肠黏膜屏障作用的影响

目的 探讨高压氧治疗对严重烧伤大鼠肠黏膜屏障作用的影响。方法 健康成年雄性SD大鼠45只，采用数字表法，随机分为假烫组(n=16)、烧伤组(n=14)和烧伤+HBO治疗组(n=15)。采用大鼠背部30％ TBSAⅢ度烫伤模型，高压氧治疗组在烧伤后24h始，在高压氧舱0.15 MPa( 1.5ATA)压力下，每日吸入95％以上浓度的氧气1h，连续5d。各组大鼠于伤后第7天取肠黏膜组织血及下腔静脉，观察各组肠黏膜组织病理变化差别，测血浆二胺氧化酶(DAO)、D-乳酸等指标。结果 与假烫组相比，烧伤组肠黏膜出现明显病理改变，而应用高压氧治疗后，肠黏膜损伤明显减轻。烧伤后第7天大鼠血浆DAO和D-乳酸水平明显增高，而应用高压氧治疗后，上述两指标水平明显下降。结论 高压氧治疗能够对严重烧伤大鼠肠黏膜屏障起到保护作用。

Effect of hyperbaric oxygen on intestinal mucosa injury of severe burn rats

Objective To explore the effect of hyperbaric oxygen (HBO) on intestinal mucosa injury of severe burn rats. Methods Forty-five male healthy adult SD rats were randomly divided into 3 groups: the sham group(n = 16), the burn injury group(n = 14), and burn injury + HBO group(n = 15). The animals in burn injury groups sustained 3-degree burn injury on the back with a total body surface area (TBSA) of 30％.Twenty-four hours after bum injury, those in the HBO group were exposed to 95％ oxygen at a pressure of 0. 15MPa, 1 hour a day, for a succession of 5 days. On the 7th day after bum injury, blood samples from the intestinal mucosa and inferior vena cava were collected to detect differences in pathological changes in the intestinal mucosa of various groups, and serum levels of diamine oxidase (DAO) and D-lactate were also measured. Results When compared with the sham group, significant pathological changes in the intestinal mucosa could clearly be seen in the bum injury group. Following exposure to HBO, lesion in the intestinal mucosa improved obviously. On the 7th day after bum injury, levels of DAO and D-lactate increased significantly. However, after HBO treatment, the levels of DAO and D-lactate decreased significantly. Conclusions HBO seemed to have the effect of protecting the barrier of intestinal mucosa in severe burn rats.