cathepsin L及其抑制剂cystatin C在急性脱髓鞘动物模型中的表达及意义

在饲料中掺入cuprizone饲育小鼠6周,制备急性脱髓鞘动物模型。进而利用髓鞘荧光染色和原位杂交方法,检测动物胼胝体内髓鞘的脱失以及cathepsin L及其抑制剂cystatin C的表达情况。结果发现,与正常动物比较,cuprizone饲育6周后小鼠胼胝体内髓鞘脱失严重,胼胝体内cathepsin L和cystatin C的表达均显著上调。结果提示,髓鞘损伤后cathepsin L高表达可能有助于髓鞘残骸的清理,其抑制剂cystatin C的表达增强可将蛋白水解程度控制在一定范围内,这对髓鞘再生具有积极的意义。

The expression and significance of cathepsin L and its inhibitor cystatin C in acutely demyelinated animal model

Cuprizone diet C57BL/6 mice were used to establish acutely demyelinated model in the present study.In these model mice,status of demyelination in corpus callosum was detected by myelin staining.The expression of cathepsin L and its inhibitor cystatin C in corpus callosum was demonstrated by using in situ hybridization.The results showed that obvious demyelination occurred in corpus callosum following 6 weeks of exposure to cuprizone,and the expression of cathepsin L and cystatin C in corpus callosum was increased significantly.It suggests that cathepsin L up-regulation may be useful for removing the debris of myelin following acute demyelination,meanwhile,cystatin C up-regulation may contribute to preventing protein from over-hydrolysis,which is helpful for remyelination.