| 大鼠颌下腺GnRH受体基因的体外扩增及基因序列分析 |
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| 引用本文： | 姚兵,黄威权,张荣庆,王雷.大鼠颌下腺GnRH受体基因的体外扩增及基因序列分析[J].解剖学报,2001,32(1):80-82. |
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| 作者姓名： | 姚兵 黄威权 张荣庆 王雷 |
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| 作者单位： | 1. 第四军医大学组织学胚胎学教研室,西安,710032
2. 清华大学生物系,北京,100084 |
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| 基金项目： | 国家自然科学基金资助项目!( 3 9770 3 88) |
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| 摘 要： | 目的 研究大鼠颌下腺ＧｎＲＨ受体基因的存在及其基因序列。方法 从ＳＤ大鼠颌下腺提取总ＲＮＡ，用ＲＴ－ＰＣＲ法扩增ＧｎＲＨ受体基因，利用双脱氧链终止法对扩增产物进行序列测定。结果 从ＳＤ大鼠颌下腺扩增出ＧｎＲＨ受体的特异性条带，经序列分析，扩增产物的基因序列与文献报道大鼠脑垂体的完全一致。结论 大鼠颌下腺有ＧｎＲＨ受体基因的表达，能合成ＧｎＲＨ受体，是ＧｎＲＨ的靶器官，颌下腺产生的ＧｎＲＨ可作用于颌下腺的靶细胞，参与颌下腺生理功能的调节。
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| 关 键 词： | 序列分析 RT－PCR GnRH受体 大鼠 颌下腺 体外扩 |
AMPLIFICATION SEQUENCE ANALYSIS OF GnRH RECEPTOR GENE IN RAT SUBMAXILLARY GLAND |
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| YAO Bing ,HUANG Wei\|quan ,ZHANG Rong\|qing ,WANG Lei.AMPLIFICATION SEQUENCE ANALYSIS OF GnRH RECEPTOR GENE IN RAT SUBMAXILLARY GLAND[J].Acta Anatomica Sinica,2001,32(1):80-82. |
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| Authors: | YAO Bing HUANG Wei\|quan ZHANG Rong\|qing WANG Lei |
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| Institution: | YAO Bing 1,HUANG Wei\|quan 1*,ZHANG Rong\|qing 2,WANG Lei 2 |
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| Abstract: | Objective\ To study whether gonadotropin releasing hormone receptor (GnRHR)mRNA exist in rat submaxillary gland and it's gene sequence. Methods\ The total RNA isolated from rat submaxillary gland was amplified by RT\|PCR, the PCR products were identified by sequencing with Sanger's method. Results\ The specific amplified band of GnRHR mRNA was detected through agarose gel electrophoresis and gene sequence is identical to the sequence of GnRHR which has been reported in rat pituitary. Conclusion\ GnRHR can be produced by submaxillary and response for modulating biological function of submaxillary. |
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| Keywords: | Sequence analysis GnRH receptor RT\|PCR Rat submaxilla@ |
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