| HPLC法测定不同产地苦瓜根中苦瓜皂苷的含量 |
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| 引用本文： | 陈黎,陈林,申德堰,何秀丽,方建敏. HPLC法测定不同产地苦瓜根中苦瓜皂苷的含量[J]. 中国药房, 2012, 0(19): 1784-1785 |
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| 作者姓名： | 陈黎 陈林 申德堰 何秀丽 方建敏 |
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| 作者单位： | 湖北医药学院附属太和医院药学部 |
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| 摘 要： | 目的:建立测定不同产地苦瓜根中苦瓜皂苷含量的方法。方法:采用高效液相色谱法。色谱柱为Gemini C18(250mm×4.6mm,5μm),流动相为水-甲醇(45:55),流速为1.0mL·min-1,检测波长为205nm,进样量为10μL。结果:苦瓜皂苷的检测浓度在0.051～1.020mg·mL-1范围内与峰面积积分值呈良好的线性关系(r=0.9998);平均加样回收率为99.38%,RSD=1.99%(n=6)。结论:不同产地苦瓜根中苦瓜皂苷的含量有较大差别,可用此法较好地评价该品种的质量。
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| 关 键 词： | 苦瓜根 苦瓜皂苷 高效液相色谱法 含量测定 |
Content Determination of Saponins in the Root of Momordica charantia from Different Areas by HPLC |
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| CHEN Li,CHEN Lin,SHEN De-yan,HE Xiu-li,FANG Jian-min. Content Determination of Saponins in the Root of Momordica charantia from Different Areas by HPLC[J]. China Pharmacy, 2012, 0(19): 1784-1785 |
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| Authors: | CHEN Li CHEN Lin SHEN De-yan HE Xiu-li FANG Jian-min |
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| Affiliation: | (Dept.of Pharmacy,The Affiliated Taihe Hospital of Hubei Medical University,Hubei Shiyan 442000,China) |
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| Abstract: | OBJECTIVE:To determine the content of saponins in the root of Momordica charantia from different areas.METHODS:HPLC method was adopted.The determination was performed on Gemini C18(250 mm×4.6 mm,5 μm) column with mobile phase consisted of water-methanol (45:55) at the flow rate of 1.0 mL·min-1.The detection wavelength was set at 205 nm,and the injection volume was 10 μL.RESULTS:The linear range of saponins was 0.051～1.020 mg·mL-1(r=0.999 8) with an average recovery of 99.38%(RSD=1.99%,n=6).CONCLUSION:The content of saponins in the root of M.charantia from different areas varies greatly,and the method can be used for quality evaluation of the species. |
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| Keywords: | The root of Momordica charantia Saponins HPLC Content determination |
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