丹酚酸乙对乳腺癌MCF-7细胞的生长抑制及诱导凋亡研究

目的]观察丹酚酸乙对乳腺癌MCF-7细胞的生长抑制及诱导凋亡作用。方法]MTT法观察丹酚酸乙对体外培养MCF-7细胞的增殖抑制作用;Hoechst33258荧光染色法观察细胞凋亡的形态学变化;流式细胞仪检测丹酚酸乙作用于MCF-7细胞48h后细胞凋亡率和细胞周期的变化;Western Blot检测caspase-3蛋白表达的变化。结果]丹酚酸乙能明显抑制MCF-7细胞的生长,呈剂量和时间依赖性;荧光染色结果显示,丹酚酸乙与MCF-7细胞共培养24、48和72h后,细胞呈现明显的核固缩、碎裂以及凋亡小体形成等细胞凋亡现象;流式细胞仪检测结果显示,不同浓度的丹酚酸乙处理MCF-7细胞48h后,细胞凋亡率和S期细胞的比例逐渐升高;Western Blot显示,随着丹酚酸乙浓度的增加,MCF-7细胞caspase-3蛋白表达水平逐渐升高。结论]丹酚酸乙对MCF-7细胞具有明显的生长抑制和促凋亡作用,这种作用可能与上调caspase-3表达以及阻滞细胞于S期有关。

The Effect of Salvianolic Acid B on Growth Inhibition and Apoptosis Induction in Breast Cancer MCF-7 Cells

Purpose] To observe the effect of salvianolic acid B on growth inhibition and apoptosis induction in breast cancer MCF-7 cells.Methods] The effect of salvianolic acid B on inhibition of MCF-7 cell proliferation was detected by MTT assay,and the cellular morphologic apoptosis was observed by Hoechst33258 staining.Cell flow cytometry was utilized to observe the apoptosis rate and cell cycle of MCF-7 cells incubated with salvianolic acid B for 48h.Expression of caspase-3 was detected by Western Blot.Results] Salvianolic acid B obviously inhibited the growth of MCF-7 cells in a time-and dose-dependent manner.Fluorescence staining indicated that the MCF-7 cells showed some obvious apoptosis characteristics such as,nuclear condensation,fragmentation,chromatin condensation and apoptotic body formation,when the cells cultured with salvianolic acid B（0.7mg/ml） for 24,48 and 72h.FCM assay indicated that apoptosis rate and percentages of MCF-7 cells in S phase gradually increased after incubated with salvianolic acid B for 48h.Western Blot showed that the expression level of caspase-3 protein gradually rose with the increase of salvianolic acid B concentration.Conclusion] Salvianolic acid B can inhibit cell growth and induce cell apoptosis,which might relate to S stage arrested and upregalation of caspase-3 expression in MCF-7 cells.