藤黄酸对人结肠癌SW480细胞增殖及VEGFR2表达的影响

目的:观察藤黄酸对人结肠癌SW480细胞增殖和血管内皮细胞受体2(VEGFR2)表达的影响,探讨其可能抗肿瘤的作用机制。方法:以不同浓度的藤黄酸干预体外培养的人结肠癌细胞株SW480,分别于24、36、48h后,应用MTT法检测细胞增殖活性,流式细胞仪检测细胞周期分布和细胞凋亡率,Western-blot分析VEGFR2蛋白的变化。结果:藤黄酸对结肠癌细胞株SW480生长增殖具有明显抑制作用,其效应呈浓度和时间依赖性。流式细胞仪分析显示SW480细胞呈G2/M期阻滞;SW480细胞的凋亡率随藤黄酸浓度增加逐步上升。用不同浓度的藤黄酸处理SW480细胞株24h,VEGFR2蛋白的表达随药物浓度的增加而减少(P<0.01)。结论:藤黄酸能够明显抑制人结肠癌细胞株SW480的增殖,诱导SW480细胞发生G2/M期阻滞,其作用机制可能与抑制VEGFR2表达有关。

Effect of gambogic acid on the proliferation of human colon carcinoma cell SW480 and the expression of VEGFR2

Objective:To investigate the effect of gambogic acid on human Colorectal Cancer Cell Line SW480 through suppression of VEGFR2 expression.Methods Methyl thiazolyl tetrazolium(MTT) assay was used to test the changes in the proliferation of SW480 cells cultured with different concentrations of gambogic acid for 24,36 and 48h in vito.Cell-cycle kinetics and apoptosis were analyzed by flow cytometry(FCM).Western-blot was applied to examine the protein expression of VEGFR2.Results:The proliferation of SW480 cells was obviously suppressed by gambogic acid in a concentration-and time-dependent manner.The cell was arrested at G2/M and apoptosis was induced.When SW480 cells were treated with gambogic acid at the concentration of 0、0.5、1.0、4.0μg/ml,the apoptosis rates of SW480 cell were(0.17±0.08)%、(0.85±0.19)%、(7.12±1.21)%、(15.87±1.59)%,respectively.After SW480 cells were treated with 0、0.5、1.0、4.0μg/ml gambogic acid for 24h respectively,the level of VEGFR2 protein decreased as concentration increased(P<0.01).Conclusions:Gambogic acid can significantly inhibit the proliferation and induces G2/M arrest in human colon cancer cell line SW480.The effect mechanism is related to suppression of VEGFR2 expression.