| RP-HPLC法测定杜仲中杜仲醇的含量 |
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| 引用本文： | 巩卓,王耀欣,陈鑫,冯硕,车庆明,韩南银.RP-HPLC法测定杜仲中杜仲醇的含量[J].中国药房,2012(11):1023-1024. |
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| 作者姓名： | 巩卓 王耀欣 陈鑫 冯硕 车庆明 韩南银 |
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| 作者单位： | 北京大学药学院 |
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| 摘 要： | 目的:建立测定杜仲中杜仲醇含量的方法。方法:采用反相高效液相色谱法。色谱柱为Chromasail C18(250mm×4.6mm,5μm),流动相为甲醇-水(8∶92),流速为1mL·min-1,柱温为25℃,检测波长为210nm。结果:杜仲醇进样量在0.4676～5.8450μg范围内与峰面积积分值呈良好的线性关系(r=0.9999);平均加样回收率为99.04%,RSD=2.16%(n=9)。结论:本方法简单、稳定、可控性强、重复性好,可用于杜仲中杜仲醇的含量测定。
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| 关 键 词： | 杜仲 杜仲醇 反相高效液相色谱法 含量测定 |
Content Determination of Eucommiol in Eucommia ulmoides by RP-HPLC |
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| GONG Zhuo, WANG Yao-xin, CHEN Xin, FENG Shuo, CHE Qing-ming, HAN Nan-yin.Content Determination of Eucommiol in Eucommia ulmoides by RP-HPLC[J].China Pharmacy,2012(11):1023-1024. |
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| Authors: | GONG Zhuo WANG Yao-xin CHEN Xin FENG Shuo CHE Qing-ming HAN Nan-yin |
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| Institution: | (School of Pharmaceutical Sciences, Peking University, Beijing 100191) |
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| Abstract: | OBJECTIVE: To develop the method for the content determination of eucommiol in Eucommia ulmoides. METHODS: RP-HPLC method was adopted. The sample was separated on Chromasail C18(250 mm×4.6 mm,5 μm) column with methanol-water (8 ∶ 92) as the mobile phase at the flow rate of 1 mL·min-1. The column temperature was 25 ℃and the detection wavelength was set at 210 nm. RESULTS: The linear range of eucommiol was 0.467 6～5.845 0 μg(r=0.999 9) with an average recovery of 99.04%(RSD=2.16%,n=9). CONCLUSION: The method is simple, stable, controllable and repeatable, and it could be used for the content determination of encommiol in Eucommia ulmoide. |
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| Keywords: | Eucommia ulmoide Eucommiol RP-HPLC Content determination |
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