紫外分光光度法测定甘草中总黄酮的含量

目的:建立甘草总黄酮的含量测定方法。方法:以甘草苷为对照品,以10%KOH溶液为显色剂,优化显色时间、显色剂用量,并最终建立紫外分光光度法测定甘草总黄酮。结果:确定最佳的显色剂用量为1.5mL、显色时间为15min;甘草苷的进样浓度在0.012～0.030mg.mL-1范围内与吸光度呈良好的线性关系(r=0.9998),平均加样回收率为99.0%,RSD=2.60(n=9)。结论:该方法快速、简单、有效,适用于甘草总黄酮含量的测定。

Content Determination of Total Flavonoids from Radix Et Rhizoma Glycyrrhizae by UV Method

OBJECTIVE:To establish the method for the content determination of total flavonoids from Radix Et Rhizoma Glycyrrhizae.METHODS:Liquiritin and 10% KOH solution were used as substance control and color reagent to optimize color developing time and amount of color reagent.At last,UV spectrophotometry was established for the content determination of total flavonoids from Radix Et Rhizoma Glycyrrhizae.RESULTS:Optimal amount of color reagent was 1.5 mL and color developing time was 15 min;the linear range of liquiritin was 0.012～0.030 mg·mL-1(r=0.999 8) with an average recovery of 99.0%(RSD=2.60,n=9).CONCLUSION:The method is rapid,simple and effective,and it is suitable for the content determination of content determination of total flavonoids from Radix Et Rhizoma Glycyrrhizae.