清肺合剂HPLC指纹图谱研究

目的:建立清肺合剂的高效液相色谱指纹图谱。方法:色谱柱为ElitSinoChromODS-AP(250mm×4.6mm,5μm),柱温为50℃,流动相为0.01%磷酸溶液-乙腈(梯度洗脱),流速为1.0mL.min-1,检测波长为330nm。结果:建立了16批清肺合剂的对照指纹图谱,得到共有峰16个,其中对香豆酸峰为参照峰。各批次样品的指纹图谱及对照图谱间相似度高。结论:所建方法准确、简便、稳定性好,可为清肺合剂的生产和质量控制提供依据。

Study on HPLC Fingerprints of Qingfei Mixture

OBJECTIVE:To establish a HPLC fingerprint of Qingfei mixture.METHODS:The optimal condition of separation and detection were achieved on an Elite SinoChrom ODS-AP(250 mm×4.6 mm,5 μm) column with mobile phase consisted of acetonitrile and 0.01% phosphoric acid aqueous solution(gradient elution) at flow rate of 1.0 mL·min-1.The detection wavelength was set at 330 nm.RESULTS:Control fingerprints of 16 batches of Qingfei mixture were established and 16 peaks were regarded as the characteristic peaks.The peak of p-coumaric acid was appointed as the reference peak.According to the similarity,the diversity of quality among different samples was minor.CONCLUSION:Established method is accurate,simple and stable.It provides basis for the production and quality control of Qingfei mixture.