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| The receptor for β2GP Ⅰon membrane of hepatocellular carcinoma cell line SMMC-7721 is annexin Ⅱ | |
| 引用本文： | Gao PJ,Shi Y,Gao YH,Liu YW,Tan Y. The receptor for β2GP Ⅰon membrane of hepatocellular carcinoma cell line SMMC-7721 is annexin Ⅱ[J]. World journal of gastroenterology : WJG, 2007, 13(24): 3364-3368. DOI: 10.3748/wjg.v13.i24.3364 |
| 作者姓名： | Gao PJ Shi Y Gao YH Liu YW Tan Y |
| 作者单位： | Pu-Jun Gao,Yang Shi,Yan-Hang Gao,Ya-Wen Liu,Yan Tan Pu-Jun Gao,Yang Shi,Yan-Hang Gao,Department of Gastroenterology,The First Hospital of Jilin University,Changchun 130021,Jilin Province,ChinaYa-Wen Liu,Department of Epidemiology,School of Public Health,Jilin University,Changchun 130021,Jilin Province,ChinaYan Tan,Department of Center Laboratory,The First Hospital of Jilin University,Changchun 130021,Jilin Province,China |
| 基金项目： | Supported by The National Nature Science Foundation of China,No. 30070338 |
| 摘 要： | AIM： To evaluate the receptor protein which can specifically bind to β2GP Ⅰ on the membrane of hepatocellular carcinoma （HCC） cell line SMMC-7721, and to study the biological function of the receptor. METHODS： Through β2GP Ⅰ -affinity chromatography column, the peptid-polysome-mRNA complex, which can specially bind to β2GPⅠ, stayed with the column and was separated from the whole polysome of liver cells, and then eluted and collected. Using cDNA synthesis kit and cDNA PCR kit, the corresponding cDNA was obtained and sequenced. RT-PCR was used to amplify annexin Ⅱ, and flow cytometry was used to study the competitive binding of annexin Ⅱ with β2GPⅠ to SMMC-7721. RESULTS： A total of 1.1 kb of the cDNA fragment of the specific binding protein of β2GPⅠ on liver cell membrane was obtained. The sequence of cDNA shared high homology with human annexin Ⅱ（98%）. Annexin Ⅱ was expressed on the membrane of SMMC-7721, and could compete with β2GP Ⅰ for combining with SMMC-7721. CONCLUSION： The receptor for β2GP Ⅰ on membrane of SMMC-7721 cells is annexinⅡ, which might bridge HBV to infect hepatocytes. |
| 关 键 词： | 肝癌 症状 治疗方法 肝细胞 |
| 收稿时间： | 2007-03-19 |
The receptor for beta(2)GP I on membrane of hepatocellular carcinoma cell line SMMC-7721 is annexin II |
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| Gao Pu-Jun,Shi Yang,Gao Yan-Hang,Liu Ya-Wen,Tan Yan. The receptor for beta(2)GP I on membrane of hepatocellular carcinoma cell line SMMC-7721 is annexin II[J]. World journal of gastroenterology : WJG, 2007, 13(24): 3364-3368. DOI: 10.3748/wjg.v13.i24.3364 | |
| Authors: | Gao Pu-Jun Shi Yang Gao Yan-Hang Liu Ya-Wen Tan Yan |
| Affiliation: | 1. Department of Gastroenterology,The First Hospital of Jilin University,Changchun 130021,Jilin Province,China 2. Department of Epidemiology,School of Public Health,Jilin University,Changchun 130021,Jilin Province,China 3. Department of Center Laboratory,The First Hospital of Jilin University,Changchun 130021,Jilin Province,China |
| Abstract: | AIM: To evaluate the receptor protein which can specifically bind to beta(2)GP I on the membrane of hepatocellular carcinoma (HCC) cell line SMMC-7721, and to study the biological function of the receptor. METHODS: Through beta(2)GP I -affinity chromatography column, the peptid-polysome-mRNA complex, which can specially bind to beta(2)GP I, stayed with the column and was separated from the whole polysome of liver cells, and then eluted and collected. Using cDNA synthesis kit and cDNA PCR kit, the corresponding cDNA was obtained and sequenced. RT-PCR was used to amplify annexin II, and flow cytometry was used to study the competitive binding of annexin II with beta(2)GP I to SMMC-7721. RESULTS: A total of 1.1 kb of the cDNA fragment of the specific binding protein of beta(2)GP I on liver cell membrane was obtained. The sequence of cDNA shared high homology with human annexin II (98%). Annexin II was expressed on the membrane of SMMC-7721, and could compete with beta(2)GP I for combining with SMMC-7721. CONCLUSION: The receptor for beta(2)GP I on membrane of SMMC-7721 cells is annexin II, which might bridge HBV to infect hepatocytes. |
| Keywords: | β2-Glycoprotein Ⅰ Hepatocellular carcinoma cell,Human annexin Ⅱ |
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