维拉帕米对牛眼小梁细胞合成细胞外基质的影响

目的 探讨维拉帕米(Verapamil)对牛眼小梁细胞(BTMC)合成Ⅰ、Ⅲ、Ⅳ型胶原、纤维连接蛋白、透明质酸的影响。方法 采用免疫组化结合计算机图像分析和放免技术检测等于和低于0.01 mg/ml维拉帕米对BTMC合成胶原、纤维连接蛋白、透明质酸的影响。结果 0.001、0.005、0.01mg/ml维拉帕米可质量浓度依赖性抑制Ⅰ、Ⅲ型胶原、纤维连接蛋白的合成(P<0.05),而促进透明质酸的合成(P<0.05)。0.005、0.01mg/ml维拉帕米可质量浓度依赖性抑制Ⅳ型胶原(P<0.05),而0.001mg/ml维拉帕米无此作用(P>0.05)。结论 维拉帕米可通过抑制细胞外基质在小梁网异常沉积,减少房水外流阻力,有望在发病学环节上治疗原发性开角型青光眼。

The effect of verapamil on synthesis of extracellular matrix in bovine trabecular meshwork cells

Objective To study the effect of verapamil on synthesis of collagen- I , collagen-III, collagen-IV , fibronectin and hyaluronic acid in bovine trabecular meshwork cells( BTMC). Methods The effect of verapamil equal to or less than 0. 01 mg/ml on synthesis of collagen-I , collagen-III , collagen-IV, fibronectin, hyaluronic acid in BTMC were examined by immunohistochemistry with computer-aided color image analysis and radioimmunoassay respectively. Results 0. 001 ,0. 005, 0.01 mg/ml verapamil inhibited synthesis of collagen-I , collagen- III , fibronectin in a concentration-dependent manner ( P < 0. 05) ,but increased the synthesis of hyaluronic acid ( P < 0. 05 ). 0. 005 ,0. 01 mg/ml verapamil could dose-dependently inhibit synthesis of collagen-IV ( P < 0. 05 ) , but there was no significant effect on collagen-IV at the level of 0. 001 mg/ml. Conclusions Verapamil may be expected to treat primary open-angle glaucoma ( POAG) through inhibiting abnormal deposition of extracellular marix(ECM)in BTMC and decreasing the resistance to the outflow of aqueous humor subsequently.