中试防龋DNA疫苗pGJA-P/VAX免疫小鼠的实验研究

目的：评价中试防龋DNA疫苗pGJA-P/VAX诱导小鼠体内特异性免疫应答的效果。方法：分别由武汉生物制品研究所中试制备和实验室采用德国Qiagen公司去内毒素质粒大量提取试剂盒手工提取防龋DNA疫苗pGJA-P/VAX;两种方法制备的质粒分别于0周,2周经肌肉、鼻腔免疫小鼠,并以鞭毛蛋白作为黏膜佐剂与质粒同时经鼻腔免疫小鼠,PBS及空载体pVAX1作为对照。免疫前开始每两周收集小鼠血清和唾液样本,ELISA定量检测血清及唾液中特异性抗体含量。结果：中试质粒和手提质粒肌注时均能够诱导血清特异性IgG,但诱导唾液特异性sIgA的能力较为有限。在与黏膜佐剂鞭毛蛋白同时滴鼻时,中试质粒可诱导较为明显的血清特异性IgG和唾液特异性sIgA。在相同的免疫条件下,中试质粒诱导的特异性抗体水平低于手提质粒组,但二者无显著性差异。结论：中试防龋DNA疫苗pGJA-P/VAX能够诱导小鼠体内特异性体液免疫应答。

Experimental Study of the Pilot-scale Products of Anti-caries DNA Vaccine pGJA-P/VAX in Mice

Objective: To evaluate the effect of the pilot-scale products of anti-caries DNA vaccine pGJA-P/VAX on inducing specific humoral immune response in mice.Methods: Anti-caries DNA vaccine pGJA-P/VAX were prepared by pilot-scale production in Wuhan Institute of Biologic Products,or extracted and purified using EndoFree Plasmid Maxi Kit in laboratory.Balb/c mice were immunized by both plasmids at week 0 and 2 by intramuscular injection or intranasal immunization.Flagellum protein was used as mucosal adjuvant,an...