| Plastic Changes of Synapses and Excitatory Neurotransmitter Receptors in Facial Nucleus Following Facial-facial Anastomosis |
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| 作者姓名： | 陈沛 宋俊 罗凌惠 龚树生 |
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| 作者单位： | Department of Otorhinolaryngology-Head and Neck Surgery，Union Hospital，Tongji Medical College，Huazhong University of Science and Technology，Department of Otorhinolaryngology-Head and Neck Surgery，Beijing Tongren Hospital，Capital University of Medical Sciences |
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| 摘 要： | The remodeling process of synapses and neurotransmitter receptors of facial nucleus were observed. Models were set up by facial-facial anastomosis in rat. At post-surgery day （PSD） 0,7,21and 60, synaptophysin （p38）, NMDA receptor subunit 2A and AMPA receptor subunit 2 （GluR2） were observed by immunohistochemical method and semi-quantitative RT-PCR, respectively. Mean-while, the synaptic structure of the facial motorneurons was observed under a transmission electron microscope （TEM）. The intensity of p38 immunoreactivity was decreased, reaching the lowest value at PSD day 7, and then increased slightly at PSD 21. Ultrastructurally, the number of synapses in nucleus of the operational side decreased, which was consistent with the change in P38 immunoreactivity. NMDAR2A mRNA was down-regulated significantly in facial nucleus after the operation （P〈0.05）, whereas AMPAR2 mRNA levels remained unchanged （P〉0.05）. The synapses innervation and the expression of NMDAR2A and AMPAR2 mRNA in facial nucleus might be modified to suit for the new motor tasks following facial-facial anastomosis, and influenced facial nerve regeneration and recovery.
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| 关 键 词： | 神经递质受体 面神经核 突触素 面部 吻合 免疫组织化学法 兴奋性 NMDA受体亚单位 |
| 收稿时间： | 12 May 2009 |
Plastic changes of synapses and excitatory neurotransmitter receptors in facial nucleus following facial-facial anastomosis |
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| Pei Chen,Jun Song,Linghui Luo,Shusheng Gong.Plastic Changes of Synapses and Excitatory Neurotransmitter Receptors in Facial Nucleus Following Facial-facial Anastomosis[J].Journal of Zuazhong University of Science and Technology: Medical Edition,2008,28(6):714-718. |
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| Authors: | Pei Chen Jun Song Linghui Luo Shusheng Gong |
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| Institution: | 1. Department of Otorhinolaryngology-Head and Neck Surgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology. Wuhan 430022, China.
2. Department of Otorhinolaryngology-Head and Neck Surgery, Beifing Tongren Hospital, Capital University of Medical Sciences,Beijing 100730, China. |
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| Abstract: | The remodeling process of synapses and neurotransmitter receptors of facial nucleus were observed. Models were set up by facial-facial anastomosis in rat. At post-surgery day (PSD) 0, 7, 21 and 60, synaptophysin (p38), NMDA receptor subunit 2A and AMPA receptor subunit 2 (GluR2) were observed by immunohistochemical method and semi-quantitative RT-PCR, respectively. Mean-while, the synaptic structure of the facial motorneurons was observed under a transmission electron microscope (TEM). The intensity of p38 immunoreactivity was decreased, reaching the lowest value at PSD day 7, and then increased slightly at PSD 21. Ultrastructurally, the number of synapses in nucleus of the operational side decreased, which was consistent with the change in P38 immunoreactivity. NMDAR2A mRNA was down-regulated significantly in facial nucleus after the operation (P<0.05), whereas AMPAR2 mRNA levels remained unchanged (P>0.05). The synapses innervation and the expression of NMDAR2A and AMPAR2 mRNA in facial nucleus might be modified to suit for the new motor tasks following facial-facial anastomosis, and influenced facial nerve regeneration and recovery. |
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| Keywords: | facial motor neurons synapse neurotransmitter receptor excitability control |
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