60m模拟空气反复潜水对大鼠免疫功能的影响

目的:探讨60 m (700 kPa)模拟空气反复潜水对大鼠免疫功能的影响.方法:将大鼠置动物加压舱内,暴露于700 kPa空气环境下60 min,每天2次(8:00～9:00, 14:0 0～1 5:00),连续3 d.于暴露后第1、3、5 天晨断头取血及脾脏.采用流式细胞术测定外周血及脾淋巴细胞亚型,用3H-TdR掺入法测定脾淋巴细胞增殖反应,以ELISA法测定循环血浆及脾淋巴细胞经刀豆球蛋白(ConA)刺激后分泌的IL-2水平以及比色法测定血浆中超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、谷胱甘肽过氧化物酶 (GPx)、谷胱甘肽S转移酶(GST)的活力和还原型谷胱甘肽(GSH)、丙二醛(MDA)的含量.结果:经高压空气反复暴露后,外周血及脾CD3+、CD4+CD3+细胞含量及CD4+CD3+/C D8+CD3+比值均显著下降(P<0.05,P<0.01),CD8+CD3+细胞含量无明显变化;脾淋巴细胞增殖率显著下降(P<0.01);血浆及脾淋巴细胞经ConA刺激后分泌的IL-2均显著下降(P<0.05).淋巴细胞亚型变化在反复暴露后第3天即完全恢复正常( P<0.05,P<0.01),暴露后第5天仍维持于正常对照水平;脾淋巴细胞增殖率、血浆及脾淋巴细胞经ConA刺激后分泌的IL-2水平在暴露后第3天均上升(P<0.05),至第5天恢复至对照水平.结论:60 m模拟空气反复潜水对大鼠免疫功能有抑制作用,3～5 d后可恢复正常.

Effects of 60 m simulated air repetitive diving on immune functions in rats

Objective: To explore the immune function changes after 60 m repetitive air diving in rats. Methods: Adult rats were placed in an animal chamber(700 kPa air,for 60 min) twice a day(8:00 9:00, 14:00 15:00) for 3 d. Peripheral blood and spleen were sampled on the 1st, 3rd and 5th day following exposure. The subpopulations of lymphocyte in blood and spleen were identified using 3 coloured flow cytometry. 3H TdR incorporation were used to assess the proliferation rate of splenic lymphocyte. IL 2 in plasma or excreted by splenic lymphocytes after ConA stimulation were determined with ELISA. Activities of superoxide dismutase (SOD),catalase (CAT),glutathione peroxidase (GPx),glutathione S transferase (GST) and levels of glutathione (GSH),methylenedioxyamphetamine (MDA) in plasma were determined by colorimetry.Results: After hyperbaria exposure CD3 +, CD4 +CD3 + cells and the CD4 +CD3 +/CD8 +CD3 + ratio all decreased in both peripheral blood and spleen( P <0.05 and P <0.01). The percentage of CD8 +CD3 + cell didnot change. The proliferation rate of splenic lymphocyte and the IL 2 in plasma or excreted by splenic lymphocyte after ConA stimulation were all reduced ( P < 0.05 and P < 0.01). The changes in lymphocyte subpopulations all disappeared on the 3rd day after exposure ( P < 0.05) and remained normal on the 5th day. The proliferation rate of splenic lymphocyte and the IL 2 in plasma or excreted by splenic lymphocyte after ConA stimulation increased gradually on the 3rd day( P <0.05) and recovered to the control levels on the 5th day ( P < 0.01 and P <0.05). Conclusion:There exist inhibitory effects of 60 m simulated air repetitive diving on rat immune functions which can recover in 3 5 d.