BALB/c小鼠腹腔接种HBV表面S抗原疫苗引起的CTL反应

目的研究BALB/c小鼠免疫接种HBV表面S抗原疫苗(HBV small surface vaccine, S-HBsAg)能否引起特异性细胞毒T淋巴细胞(CTL)反应。方法分别给BALB/c小鼠腹腔内接种0~6 μg S-HBsAg,两周后加强一次,4周后分离小鼠脾细胞,体外用S-HBsAg特异性CTL表位多肽刺激;用特异性多肽、51Cr标记的P815细胞作为靶细胞;4 h51Cr释放实验检测CTL反应。结果分别接种0、0.65、1.25、2.5、5μg S-HBsAg的小鼠,其脾淋巴细胞CTL特异性释放率分别为31.21%±9.23%、42.36%±19.32%、91.21%±22.97%、69.25%±24.13%、51.49%±21.661%;只接受一次免疫接种的小鼠,其脾淋巴细胞CTL特异性释放率分别为27.34%±14.25%、32.27%±15.35%、56.28%±24.35%、44.34%±18.65%、40.76%±56%。结论BALB/c(H-2d)小鼠腹腔内接种S-HBsAg引起剂量依赖性特异性CTL反应,加强免疫能够提高CTL反应。

Cytoxic T lymphocyte responses to hepatitis B virus small surface antigen in BALB/c mice

Objective To observe cytotoxic T lymphocytes (CTL) responses induced by immunization of mice with hepatis B small surface antigen (S-HBsAg). Methods BALB/c(H-2d) mice were injected intraperitoneally with S-HBsAg at the doses ranging from 0-5 μg with a booster injection 2 weeks later. Four weeks after the booster immunization, the specific cytotoxic reactivity of the spleen lymphocytes isolated from the immunized mice, following stimulation by S-HBsAg specific CTL epitope peptide in vitro, with 51Cr-labeled P815 cells treated with the peptide was observed. Results The percentage of specific release of 51Cr in mice immunized with S-HBsAg at the doses of 0, 0.65, 1.25, 2.5, and 5 μg was 31.21%±9.23%, 42.36%±19.32%, 91.21%±22.97%, 69.25%±24.13% and 51.49%±21.661% respectively. In mice receiving the only primary vaccination, the corresponding percentage was 27.34%±14.25%, 32.27%±15.35%, 56.28%±24.35%, 44.34%±18.65% and 40.76±56% respectively. Conclusion Immunization with S-HBsAg efficiently elicits CTL response in BALB/c mice in a dose-dependent manner and enhanced by booster immunization.