前列腺癌bcl-2及p53蛋白表达水平的研究

为了研究前列腺癌bcl 2及 p5 3蛋白的表达水平及其与前列腺癌发生发展的关系 ,应用LSAB法检测了 2 8例前列腺癌患者的bcl 2及 p5 3蛋白的表达水平。结果显示 ,bcl 2及p5 3蛋白表达的阳性率分别为 4 2 .9%、2 8.6 % ,均高于良性前列腺增生的阳性率 ,但无统计学差异 (P >0 .0 5 )。在前列腺癌中 ,低分化组bcl 2的表达水平明显高于高及中分化组 (P <0 .0 5 ) ,临床分期D期明显高于B及C期 (P <0 .0 5 ) ;p5 3的表达水平在上述各组及各期之间均无统计学差异 (P >0 .0 5 )。提示前列腺癌bcl 2蛋白的表达异常增高 ,可能与前列腺癌的发生发展有关 ,检测bcl 2蛋白的表达水平可以作为前列腺癌诊断及预后判断的一项参考指标

Expression of bcl-2 and p53 Proteins in Prostatic Carcinoma

Expression of bcl-2 and P53 proteins in twenty-eight cases of prostatic carcinoma and ten cases of benign hyperplasia of prostate was detected by immunohistochemical LSAB assay to study the relationship between these gene products and the pathogenesis of prostatic carcinoma. It was found that bcl-2 and p53 positive rates in prostatic carcinoma were 42.9% and 28.6% respectively, both of which were not significantly higher than those in benign hyperplasia of prostate statically, but a tendancy of their increasing expression in prostatic carcinoma could be seen. The expression of bcl-2 in the group of poor differentiation was significantly higher than that in the well-differentiated group (P<0.05). When compared in terms of clinical stages, the bcl-2 expression at stage D was significantly higher than that at stage B or C (P<0.05). There existed a tendency of increase in p53 expression in prostatic carcinoma, but it was not significantly higher than that in benign hyperplasia of prostate statistically. When the poorly differentiated group and well-differentiated group were compared, there was no statistically significant difference, and the comparison based on different clinical stages didn't show significant difference in p53 expression between stage and Stage B or C. It is suggested that bcl-2 protein expression increases in prostatic carcinoma and is, to some extent, involved in the pathogenesis of the disease. It may be concluded that bcl-2 protein assay can be used as an index of diagnosis and prognosis of the disease. The relationship between p53 expression and prostatic carcinoma remains to be learned.