| 米非司酮调控卵巢癌细胞表达葡萄糖神经酰氨合成酶并逆转其多药耐药性 |
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| 引用本文： | 刘颖,王丽莉. 米非司酮调控卵巢癌细胞表达葡萄糖神经酰氨合成酶并逆转其多药耐药性[J]. 南方医科大学学报, 2008, 28(9): 1727-1730 |
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| 作者姓名： | 刘颖 王丽莉 |
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| 作者单位： | 广东省人民医院妇产科,广东,广州,510080;南方医科大学珠江医院妇产科,广东,广州,510282 |
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| 摘 要： | 目的 观察米非司酮体外逆转COC1/DDP细胞对顺铂的多药耐药性(MDR)以及对细胞葡萄糖神经酰氨合成酶(GCS)表达的影响,初步探讨其增敏机制.方法 以米非司酮为MDR修饰剂,MTT法检测米非司酮对COC1/DDP的增敏效应.RT-PCR技术分析耐药株COC1/DDP的MDR逆转前后以及亲本株COC1细胞的GCS在mRNA水平的表达.结果 米非司酮增强COC1/DDP细胞对DDP敏感性;与COC1相比,COC1/DDP细胞的GCS在mRNA水平的表达增强;米非司酮使GCS表达降低,效应呈剂量依赖关系.结论 无毒性剂量米非司酮可以在体外增加卵巢癌COC1/DDP细胞对DDP的敏感性.作用机制与抑制GCSmRNA表达有关.
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| 关 键 词： | 米非司酮 卵巢肿瘤 葡萄糖神经酰胺合成酶 多药耐药 |
Mifepristone modulates glucosylceramide synthase expression and reverse multidrug resistance in ovarian cancer cells |
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| LIU Ying,WANG Li-li. Mifepristone modulates glucosylceramide synthase expression and reverse multidrug resistance in ovarian cancer cells[J]. Journal of Southern Medical University, 2008, 28(9): 1727-1730 |
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| Authors: | LIU Ying WANG Li-li |
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| Affiliation: | LIU Ying1,WANG Li-li2 1Department of Obstetrics , Gynecology,Guangdong Provincial People's Hospital,Guangzhou 510080,China,2Zhujiang Hospital,Southern Medical University,Guangzhou 510282 |
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| Abstract: | Objective To evaluate the effect of mifepristone in reversing multidrug resistance(MDR) and modulating glucosylceramide synthase(GCS) mRNA expression in human ovarian cancer COC1/DDP cells.Methods MDR cell line COC1/DDP was treated with mifepristone at different concentrations.The alterations in the chemosensitivity of the cells to cisplatin(DDP) were evaluated by MTT assay.GCS mRNA expression in COC1/DDP cells were detected using RT-PCR before and after mifepristone treatment.Results The expression level o... |
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| Keywords: | mifepristone ovarian carcinoma glucosylceramide synthase multidrug resistance |
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