| CD4+T细胞在痢疾疫苗诱导小鼠肠粘膜免疫应答中的作用 |
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| 引用本文： | 张亚东,高杰英,彭虹.CD4+T细胞在痢疾疫苗诱导小鼠肠粘膜免疫应答中的作用[J].细胞与分子免疫学杂志,1999,0(2):115-117. |
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| 作者姓名： | 张亚东 高杰英 彭虹 |
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| 作者单位： | 军事医学科学院微生物流行病学研究所!北京100850 |
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| 摘 要： | 以本室构建的双价痢疾候选疫苗株ＦＳ５４１６ 作为口服免疫原，检测了小鼠ＰＰ 结（Ｐｅｙｅｒ ，ｓ ｐａｔｃｈｅｓ） 中Ｔ 淋巴细胞的体外增殖反应， 观察了腹腔注射抗Ｌ３Ｔ４ ｍ Ａｂ 对小鼠肠粘膜部位产生特异性Ｉｇ 分泌细胞的影响。结果表明：①小鼠口服免疫后，ＰＰ 结中的ＣＤ４ ＋ Ｔ 细胞可产生明显的特异性增殖反应：②选择性地删除ＣＤ４ ＋ Ｔ 细胞后，小鼠肠粘膜无抗原特异性Ｉｇ 分泌细胞产生。以上提示，小鼠肠粘膜对痢疾疫苗的免疫应答需要ＣＤ４ ＋ Ｔ 细胞的参与。
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| 关 键 词： | CD~ T细胞 增殖反应 抗L3T4mAb 痢疾疫苗 |
Role of CD4~ T cells in mucosal immune respones of mice after oral immunization with the bivalent Shigella vaccine candidates |
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| Zhang Ya dong,Gao Jie ying,Peng
Hong,Xing Li,Zeng Guan fu.Role of CD4~ T cells in mucosal immune respones of mice after oral immunization with the bivalent Shigella vaccine candidates[J].Journal of Cellular and Molecular Immunology,1999,0(2):115-117. |
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| Authors: | Zhang Ya dong Gao Jie ying Peng
Hong Xing Li Zeng Guan fu |
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| Institution: | Zhang Ya dong,Gao Jie ying,Peng
Hong,Xing Li,Zeng Guan fu
(Laboratory of Immunology, Institute of Microbiology and Epidemiology,Academy of Military
Medical Sciences ,Beijing 100850) |
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| Abstract: | After the mice were orally immunized with live attenuated bivalent hybrid strain FS-5416 of S.flexneri 2a and S.sonnei,the role of CD4 T cells in Peyer's patches was studied by T cell proliferation assay in vitro and Ig isotype specific BA ELISPOT assay following selective depletion of CD4 T cells with monoclonal anti-L3T4 mAb.The CD4 T cells in peyer's patches produced strong proliferation respones after oral immunization,and anti L3T4 mAb treatment weekly resulted in a significant decrease of the antigen specific lg producing cells compared with controls.These results indicate a important role of CD4 T cells in mucosal immune responses. |
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| Keywords: | CD4 T cells cell proliferation anti L3T4 mAb Shigella vaccine |
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