| 吗啡成瘾性大鼠脑内核团神经细胞[Ca 2+]i变化的研究 |
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| 引用本文： | 王林,傅先明,钱若兵,牛朝诗,汪业汉,鲁明. 吗啡成瘾性大鼠脑内核团神经细胞[Ca 2+]i变化的研究[J]. 立体定向和功能性神经外科杂志, 2007, 20(2): 84-87 |
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| 作者姓名： | 王林 傅先明 钱若兵 牛朝诗 汪业汉 鲁明 |
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| 作者单位： | 230001,合肥,安徽医科大学附属省立医院神经外科;230001,合肥,安徽医科大学附属省立医院神经外科;230001,合肥,安徽医科大学附属省立医院神经外科;230001,合肥,安徽医科大学附属省立医院神经外科;230001,合肥,安徽医科大学附属省立医院神经外科;230001,合肥,安徽医科大学附属省立医院神经外科 |
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| 摘 要： | 目的 研究吗啡成瘾对大鼠脑内与成瘾相关核团神经细胞[Ca 2 ]i的影响.方法 将50只SD大鼠随机分成吗啡成瘾组和对照组,观察成瘾后的戒断症状;应用对Ca2 敏感的探针Fluo-3与Ca2 络合后被激光激发发出荧光的特性,利用激光共聚焦显微镜观察伏核、海马、内侧额前皮质神经细胞[Ca 2 ]i变化,并用图像分析软件进行处理.结果 吗啡成瘾大鼠脑内伏核、海马及内侧额前皮质神经细胞[Ca 2 ]i和对照组比较明显增高(P<0.01).结论 长期使用吗啡可明显增高伏核、海马和内侧额前皮质神经细胞[Ca 2 ]i.
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| 关 键 词： | 吗啡成瘾 钙离子 伏核 海马 激光共聚焦显微镜 |
| 文章编号： | 1008-2425（2007）02-0084-04 |
| 收稿时间： | 2006-11-16 |
| 修稿时间： | 2006-11-16 |
The changes of neuroal intracellular free calcium in brain regions of morphine-addicted rats |
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| Wang Lin , Fu Xianming , Qian Ruobing ,et al.. The changes of neuroal intracellular free calcium in brain regions of morphine-addicted rats[J]. Chinese Journal of Stereotactic and Functional Neurosurgery, 2007, 20(2): 84-87 |
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| Authors: | Wang Lin Fu Xianming Qian Ruobing et al. |
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| Affiliation: | Department of Neurosurgery , The Affiliated Provincial Hospital of Anhui Medical University, He fei, 230001 |
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| Abstract: | Objective To investigate the effects of morphine on the changes of neuroal intracellular free calcium in brain regions of rat.Methods 50 male Spraqui-Dawlay rats were randomly assigned to experimental or control groups.Changes of i in nucleus accumbens,hippocampus and medial prefrontal cortex were detected with laser scanning confocal microscope(LSCM) by using Ca2 -sensitive dye fluo-3 as a calcium fluorescent probe.Results i in the nucleus accumbens,hippocampus and medial prefrontal cortex were increased in morphine-addicted rats than that of control(P<0.05).Conclusion Long-term morphine exposure can lead to damage in different brain regions and increase of neuroal i. |
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| Keywords: | Morphine-addicted i Nucleus accumbens Hippocampus Laser scanning confocal microscope |
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