昆明山海棠提取物对异基因骨髓移植小鼠调节性T细胞的影响

目的 研究昆明山海棠（THH）提取物减轻异基因骨髓移植小鼠移植物抗宿主病（GVHD）作用及可能机制。

Effects of Tripterygium hypoglaucum (level) Hutch Extraction on Regulatory T Cells in Mice after Allogeneic Bone Marrow Transplantation

Objective To study the effect of Tripterygium hypoglaucum (level) Hutch (THH) extraction in alleviating the graft-versus-host disease (GVHD) in mice after allogeneic bone marrow transplantation and to explore its mechanism. Methods Mixture of 2×10~7 bone marrow cells and 2×10~7 spleen cells obtained fromC57BL/6 mouse were transplanted into the myelo-ablative irradiated inbred BALB/c mouse (H-2~d). The experi-mental mice were designed into 4 groups: the control group (A) ,the Cyclosporine A (CsA) group (B) ,the THH group (C),and the CsA + THH group (D). Condition of GVHD and transplanted chimera were observed, per-centage of CD_4~+ CD_(25)~+ T cell in peripheral blood were detected by flow cytometry, and the expression of Foxp3 mR-NA in splenocyte at different time points were determined by real-time FQ-PCR. Results CD_4~+ CD_(25)~+ T cell in Group B, C and D increased from the 10th day,peaked on the 15th day, descended after then,but the level in Group D maintained higher than that in the other three groups (P <0.05). Expression of Foxp3 mRNA in recipi-ents' splenocytes increased gradually,reached peak on the 15th day and followed with gradual decreasing, the highest peak and the slowest decreasing was shown in Group D,so the level was still rather higher in Group D on the 30th day. The survival time in the three treated group (B, C and D) were all longer than that in the control group A (P <0.05). GVHD signs and pathologic changes in the three treated groups were milder than in the con- trol group. Allogeneic chimerism rates (H-2~d) in all groups were over 95%. Conclusion THH could prevent GVHD in mice after allogeneic bone marrow transplantation, and prolong their survival time, the mechanism is possibly related with its immunosuppressive effect in raising CD_4~+ CD_(25)~+ T cells and promoting the Foxp3 mRNA ex-pression. THH combining with small dose CsA shows synergic effect in preventing GVHD.