几种不同方法制备恶性疟原虫基因组DNA的比较

目的　比较３ 种不同的基因组 Ｄ Ｎ Ａ 提取方法制备恶性疟原虫基因组 Ｄ Ｎ Ａ 的效果。方法　分别采用酚－ 氯仿－ 异戊醇抽提法、 Ｗｉｚａｒｄ 基因组 Ｄ Ｎ Ａ 纯化试剂盒和磷酸钠盐溶液， 分离提取恶性疟原虫基因组 Ｄ Ｎ Ａ， 通过 Ｄ Ｎ Ａ 含量测定和 Ｐ Ｃ Ｒ 鉴定， 比较它们的提取效果。结果　前两种方法制备恶性疟原虫基因组 Ｄ Ｎ Ａ 的效果相仿， 纯度较高； 第三种方法最为简便， 所得的 Ｄ Ｎ Ａ 含量明显高于前两种方法， 但其纯度较低， 蛋白质含量最高。结论　３ 种方法各有优缺点， 第３ 种方法简便、快捷、经济， 更有实用价值。

EFFECT COMPARISON OF THE THREE DIFFERENT METHODS FOR THE ISOLATION OF GENOMIC DNA OF PLASMODIUM FALCIPARUM

AimTo compare the effect of three different genomic DNA isolating methods in the preparation of plasmodium falciparum genomic DNA.Methods Three methods were(1)phenol/chloroform/isoamyl alcohol,(2)Wizard Genomic DNA Purification Kit,and(3)sodium phosphate solution.DNA content determination and PCR technique were applied to compare the isolating effect.ResultsThe first two methods had a similar effect with a higher purity.The third method gave a higher content of DNA,but also had a higher protein content.ConclusionThe third method is sinple,rapid and low-costed,which is of practical value.