精子蛋白质组的研究进展

蛋白质组学及其技术是新世纪最具有价值的生命科学研究手段之一。它标志着生命科学已经进入了后基因组时代。双向凝胶电泳和质谱分析方法在蛋白质组学中的成功运用,创造出更受研究者欢迎的新研究策略,并提高了研究者探索未知领域的效率。将其运用在精子蛋白质的研究后,已经取得了不少令人惊喜的成果。本文在简要概括蛋白质组及蛋白质组学的相关信息后,着重介绍近年全精子蛋白的研究情况,获能相关精子蛋白,精卵结合相关精子蛋白,以及蛋白质组学及技术在精子免疫方面的研究与应用。

Update of the Researches on Sperm Proteome

Proteomics and potemic techniques are among the most valuable approaches in the research of life science in this new century, marking the beginning of a post-genome era. Two-dimensional electrophoresis and mass spectrometry, applied as key techniques in proteomic research, have given rise to new research strategies and improved the efficiency of researchers in exploring the unknown field. Its introduction into the exploration of spermatozoal proteins has given us so many pleasant surprises. This review presents some essential information about proteomics and two-dimensional electrophoresis and mass spectrometry, with a brief introduction of the recent progress in the researches on human sperm proteome, capacitation-related sperm proteins, sperm-egg interaction-related proteins, and sperm-immunity which has made great senses in the clinical problems.