实验性肝癌中比较槐耳与IL-2对PTEN和IL-2R阳性细胞的影响

目的　比较槐耳清膏与IL - 2对抑癌基因PTEN表达和IL - 2R阳性细胞的影响。方法　应用二乙基亚硝胺 (DENA)制备 4 0只大鼠肝癌模型 ,分为 5组 ,对照组 (G1)、单纯诱癌组 (G2 )、诱癌加槐耳清膏组 (G3)、诱癌加 9周后槐耳清膏组 (G4 )和诱癌加 9周后IL - 2组(G5 )。于实验第 2 0周处死动物取肝组织 ,用RT -PCR检测PTEN表达 ,免疫组化检测IL - 2R阳性细胞 ,并通过计算机图像分析系统分别比较各组中PTEN的表达量和IL - 2R阳性细胞数。结果　G3,G4 ,G5肝癌的形成率明显降低 ,肝内IL - 2R阳性的活化T细胞数G3和G5显著高于其他各组 (P均 <0 .0 1)。在G5中 ,无癌形成的肝内IL - 2R阳性细胞数显著高于有癌形成者。PTEN相对表达量G3显著高于G2 (P <0 .0 1)。结论　槐耳清膏和IL - 2均能增加肝内IL - 2R阳性细胞数 ,增强体内细胞免疫功能 ,有效抑癌 ;槐耳清膏还能抑制抑癌基因PTEN的缺失而发挥抑癌作用 ,槐耳抗癌的应用前景将优于IL - 2。

Effect of PS-T and IL-2 on PTEN and IL-2R positive cells in experimental hepatic carcinoma

Objective It is to compare the effects of PS-T and IL-2 on the expression of PTEN and IL-2R positive cells.Methods 40 hepatic carcinoma model rats were prepared with DENA and divided into control group (G1), carcinoma inducing group (G2), carcinoma inducing and PS-T group (G3), carcinoma inducing and PS-T after 9 weeks group (G4) and carcinoma inducing and IL-2 after 9 weeks group (G5). Then all the rats were killed at the twentieth week and the liver tissues were taken out. The expression of PTEN was determined by RT-PCR. The IL-2R positive cells were determined by immunohistochemistry. Then the expression quantities of PTEN and the numbers of IL-2R positive cells in different groups were compared with computer image analyzing system.Results The hepatic carcinoma forming rates of G3, G4 and G5 were significantly decreased. The numbers of hepatic IL-2R positive cells in G3 and G5 were significantly higher than that in other groups (P<0.01). The amount of IL-2R positive cells in liver without carcinoma in G5 was significantly higher than that in liver with carcinoma. The expression quantity of PTEN in G3 was significantly higher than that in G2 (P<0.01).Conclusion Both PS-T and IL-2 can increase the numbers of IL-2R positive cells in liver, strengthen body cell immunology and restrain cancer effectively. PS-T can also restrain cancer by restraining the deletion of the anti-carcinoma gene PTEN. The anti-carcinoma foreground of PS-T is better than that of IL-2.