RP-HPLC法同时测定淫羊藿自乳化软胶囊中朝藿定A,B,C与淫羊藿苷成分的含量

目的:建立淫羊藿自乳化软胶囊中4种淫羊藿黄酮类成分的含量测定方法。方法:采用反相高效液相色谱法,色谱柱为Agilent Eclipse XDB-C18柱(250 mm×4.6 mm,5μm);流动相为水-乙腈(71∶29),检测波长270 nm,流速1.0 mL.min-1,柱温30℃。结果:朝藿定A,B,C和淫羊藿苷分别在0.952～9.52μg.mL-1,0.992～9.92μg.mL-1,0.974～9.74μg.mL-1,4.96～49.6μg.mL-1浓度范围内与峰面积呈良好的线性关系,其线性回归方程分别为:C=0.044 8A+0.410 6(r=0.999 9);C=0.029 9A+0.270 4(r=0.999 9);C=0.040 8A+0.270 4(r=0.999 9);C=0.028 3A+0.276 6(r=0.999 9),加样回收率(n=9)分别为100.1%;99.3%;100.3%和99.7%。结论:该方法操作简便、快速,准确度高,重复性好,稳定可靠,可作为淫羊藿自乳化软胶囊质量控制的方法。

An RP-HPLC determination of epimedin A,B,C and icraiin in self-emulsifying soft capsules of Epimedium brevicornum extracts

Objective: To develop a RP-HPLC method for determination of four flavonoids in self-emulsifying soft capsules of Epimedium brevicornum extracts.Methods: The determination was performed on an Eclipse XDB-C18 column(250 mm×4.6 mm,5 μm) at 30 ℃ and a flow rate of 1.0 mL·min-1.The mobile phase was water-acetonitrile(71∶29).The detection wavelength was set at 270 nm.Results: The linear correlation equations for epimedin A,B,C and icraiin were C=0.044 8A+0.410 6(r=0.999 9),C=0.029 9A+0.270 4(r=0.999 9),C=0.040 8A+0.270 4(r=0.999 9),and C=0.028 3A+0.276 6(r=0.999 9),respectively.A good linearity was obtained over the ranges of 0.952~9.52 μg·mL-1,0.992~9.92 μg·mL-1,0.974~9.74 μg·mL-1,and 4.96~49.6 μg·mL-1,respectively.The recoveries were 100.1%,99.3%,100.3% and 99.7%.Conclusion: The method is simple and suitable to control the quality of self-emulsifying soft capsules of Epimedium brevicornum extracts.