不同产地叶下珠药材中总酚含量测定

目的:建立不同产地叶下珠药材中总酚的含量测定方法.方法:以柯里拉京为对照品,三氯化铁-铁氰化钾为显色剂,检测波长744 nm,采用UV测定11个不同产地叶下珠药材中总酚的含量.结果:柯里拉京在0.604 ～ 3.02 mg·L-1线性关系良好(r =0.999 1),平均加样回收率99.79％,RSD 2.72％.不同产地叶下珠药材中总酚质量分数5.00％ ～ 12.16％.结论:该方法简单准确、重复性好,可用于叶下珠药材中总酚含量的测定.

Determination of Total Phenols from Phyllanthus urinaria in Different Locations

Objective:To establish a method for determination of total phenols from Phyllanthus urinaria in different locations. Method:Total phenols was determined by UV-Vis spectrophotometer with corilagin as reference substance and FeCl₃-K₃Fe(CN)₆] as chromogenic agent,the detection wavelength was at 744 nm. Result:A good linear relationship of corilagin was achieved in the range of 0.604-3.02 mg·L^-1 (r=0.999 1),the average recovery was 99.79% with RSD of 2.72%(n=6).The content of total phenols from P. urinaria in different locations was different from 5.00% to 12.16%. Conclusion:This established method was simple,accurate and stable which was suitable for determination the content of total phenols from P. urinaria.