| 大鼠肝叶切除后肝细胞再生相关蛋白的蛋白质组学分析 |
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| 引用本文： | 陈胤,陈平,何中林,王晓枫,还勇为. 大鼠肝叶切除后肝细胞再生相关蛋白的蛋白质组学分析[J]. 第三军医大学学报, 2008, 30(12): 1144-1147 |
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| 作者姓名： | 陈胤 陈平 何中林 王晓枫 还勇为 |
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| 作者单位： | 第三军医大学大坪医院野战外科研究所肝胆外科,重庆,400042;第三军医大学大坪医院野战外科研究所肝胆外科,重庆,400042;第三军医大学大坪医院野战外科研究所肝胆外科,重庆,400042;第三军医大学大坪医院野战外科研究所肝胆外科,重庆,400042;第三军医大学大坪医院野战外科研究所肝胆外科,重庆,400042 |
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| 摘 要： | 目的 通过对肝叶切除后6、24 h的肝细胞与正常肝细胞的蛋白质组分析,筛选出与肝细胞再生相关的蛋白质.方法 选择雄性SD大鼠9只(体质量200 g),分3组,6 h组、24 h组和对照组,每组3只,建立大鼠70%肝叶切除模型.6 h组在肝叶切除后6 h分离肝细胞,24 h组在肝叶切除后24 h分离肝细胞,对照组为正常肝细胞.裂解液裂解细胞提取总蛋白,运用蛋白组学相关技术分析3组细胞的蛋白组学差异.结果 总共得到47个差异蛋白质点,鉴定出42个.结论 我们的数据显示:24 h组与对照组和6 h组都有重叠,而6 h组和对照组重叠部分较少,推测其可能的原因为6 h组肝细胞处于肝再生的启动阶段,与对照组差异较大,而24 h组,肝再生已启动,各种维持肝细胞正常功能的蛋白质也开始表达.
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| 关 键 词： | 肝切除 肝再生 蛋白组学 二维电泳 肽质量指纹谱 |
| 文章编号： | 1000-5404(2008)12-1144-04 |
| 修稿时间： | 2007-11-25 |
Proteomic analysis of rat hepatocyte regeneration related proteins after liver resection |
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| CHEN Yin,CHEN Ping,HE Zhong-lin,WANG Xiao-feng,HUAN Yong-wei. Proteomic analysis of rat hepatocyte regeneration related proteins after liver resection[J]. Acta Academiae Medicinae Militaris Tertiae, 2008, 30(12): 1144-1147 |
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| Authors: | CHEN Yin CHEN Ping HE Zhong-lin WANG Xiao-feng HUAN Yong-wei |
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| Affiliation: | CHEN Yin,CHEN Ping,HE Zhong-lin,WANG Xiao-feng,HUAN Yong-wei (Department of Hepatobiliary Surgery,Institute of Surgery Research,Daping Hospital,Third Military Medical University,Chongqing 400042,China) |
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| Abstract: | Objective To screen proteins related with liver regeneration by studying the proteomic difference between the hepatocytes 6 h and 24 h after the 70% hepatotectmy and the normal ones.Methods Nine male SD rats(weight 200 g) were selected and randomly divided into 3 groups: the control group,the 6 h group and the 24 h group, each one with 3 rats.The rats 70% hepatectomy model was built.The hepatocytes in the control group were immediately isolated from the rat liver and those in the 6 h group and 24 h group we... |
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| Keywords: | hepatotectomy liver regeneration proteomics Two-dimensional electrophoresis peptide mass fingerprint |
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