Potential Role of Immunodiagnosis for Pulmonary Tuberculosis Using Induced Sputum Cells |
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Authors: | Doosoo Jeon Seung Eun Lee Woo Hyun Cho Byung Hee Lee Yun Seong Kim Ji-Eun Lee Eun-Soon Son Ye-Jin Lee Min-Sun Hong Seok-Yong Eum |
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Institution: | 1Department of Internal Medicine, Pusan National University School of Medicine, Yangsan, Korea.;2International Tuberculosis Research Center, Changwon, Korea. |
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Abstract: | PurposeTo evaluate the diagnostic utility and predictors for determinate results of an enzyme-linked immunospot assay using induced sputum cells (IS ELISPOT) for a rapid diagnosis of pulmonary tuberculosis (TB).ResultsA total of 43 subjects, including 25 with TB (TB group) and 18 with non-TB disease (non-TB group) were enrolled. Results of IS ELISPOT were determinate in only 17/43 (39%) subjects, but all of determinate results were consistent with the final diagnosis. Of the 43 sputum samples, 11 (26%) were inadequate to perform IS ELISPOT. Of 32 adequate sputum samples, the proportion of determinate results was significantly higher in the TB group (75%, 15/20) than in the non-TB group (17%, 2/12) (p=0.002). The status of active TB was a unique predictor but smear positivity was not a significant predictor for determinate results. In addition, sensitivity of IS ELISPOT (75%, 9/12) in smear negative TB was higher than that of TB-polymerase chain reaction (25%, 3/12).ConclusionIS ELISPOT showed relatively high diagnostic value and accuracy in the TB group, independent of smear positivity. IS ELISPOT may provide additional diagnostic yield for microbiological tools in the rapid diagnosis of smear-negative TB. |
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Keywords: | Tuberculosis induced sputum immunodiagnosis enzyme-linked immunospot assays |
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