HPLC测定御感袋泡茶中葛根素和黄芩苷的含量

 目的：采用高效液相色谱法测定黄芩、葛根及其制剂御感袋泡茶中黄芩苷和葛根素的含量，评价该制剂的质量。方法与结果：黄芩苷测定以KYWG-C₁₈为固定相，0.5%磷酸-甲醇-N，N-二甲基甲酰胺(13∶10∶1)为流动相，UV检测波长为276 nm，黄芩苷在0.1～0.01 mg/ml浓度范围与峰面积呈直线关系，回归方程为Y=0.0018+9.95×10^-8X，r=0.999 6,平均回收率为102.8%，WTBX]RSDWTBZ]为1.86%(WTBX]nWTBZ]=5)。葛根素测定以KYWG-C₁₈为固定相，水-甲醇-N，N-二甲基甲酰胺(40∶10∶3)为流动相，UV检测波长为250 nm，葛根素在0.2～0.020 mg/ ml浓度范围与峰面积呈直线关系，回归方程为Y=1.5×10^-4+1.09×10^-7X，r=0.999 9,平均回收率为101.7%，RSD为1.62%(n=5)。结论：本法简便、快速，结果可靠。

Determination the contents of baicalin and puerarin in Yugan Daipaocha by HPLC

OBJECTIVE:To evaluate the quality of baicalin in Scutellaria baicalensis Georgi,puerarin in Puerarisa lobata(Willd.) is Yagan Baipaocha by high performence liquid chromatgraphy.METHOD:Baicalin was separated on KYWG-C₁₈ column and detected at 276 nm,by using 5 g/L phosphoric acid-methanol-N,N-dimethylformamide(13∶10∶1) as mobile phase.RESULTS:Puerarin was separated on KYWG-C₁₈ column and detected at 250 nm by using water-methanol-N,N-dimethylformamide(40∶10∶3) as mobile phase.There was a good linearity from 0.1 to 0.01 mg/ml(r=0.999 6,Y=1.8×10^-3+9.95×10^-8X) in separation of baicalin.The average recovery was 102.8% and the relative standard deviation 1.86%(n=5).There was a good linearity from 0.2 to 0.020 mg/ml(r=0.999 9,Y=1.5×10^-4+1.09×10^-7X〖WTBZ〗) in separation of puerarin.The average recovery was 101.7% and the relative standard deviation 1.62%(n=5).CONCLUSION:This method is simple and fast,and the results are reliable.