Human mitochondrial glycerol phosphate dehydrogenase (GPDM) isozymes |
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| Authors: | M A SHAW Y H EDWARDS D A HOPKINSON |
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| Institution: | M.R.C. Human Biochemical Genetics Unit, Galton Laboratory University College London, 4 Stephenson Way, London NW1 2HE |
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| Abstract: | - 1 A hydrophobic/phospholipid electrophoretic system has been devised which makes possible the analysis of human mitochondrial glycerol phosphate dehydrogenase (E.C. 1.1.99.5, GPDM.
- 2 GPDM has a wide tissue distribution in both adult and foetal life and is active in cultured lymphoblastoid cells and fibroblasts but is absent from red cells.
- 3 The solubilization procedure does not significantly alter the kinetic properties of the enzyme (Kmα-glycerophosphate = 0.04-0.07 m , Km PMS = 0.19-0.35 mm ) but the soluble form is less thermostable.
- 4 Comparisons of physicochemical characteristics, tissue distribution and coenzyme requirement point to a separate genetic determination and low level of evolutionary relatedness between GPDM and its cytosolic counterpart GPDs.
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