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| 表达HPA抗原的细胞株稳定性及应用的研究 | |
| 引用本文： | 申卫东,黎海燕,李丽兰,刘学军,吴国光. 表达HPA抗原的细胞株稳定性及应用的研究[J]. 现代免疫学, 2010, 0(1) |
| 作者姓名： | 申卫东 黎海燕 李丽兰 刘学军 吴国光 |
| 作者单位： | 南宁中心血站南宁输血研究所; |
| 基金项目： | 南宁市科研资助项目(200801023C) |
| 摘 要： | 建立血小板特异性抗原永生化细胞株,为血小板免疫学及血小板交叉配型提供永久的研究材料。采用EB病毒(Ep-stein-Barr virus)转化技术,把携带血小板特异性稀有抗原外周血淋巴细胞转化成永生化淋巴母细胞株。结果:成功地建立了13个稀有血小板特异性稀有抗原建立了永久细胞株,所有的细胞株传代、冻存和复苏的成功率为100%,细胞传至30代没有发现基因突变。由所建的细胞株抽提的DNA样本,分发给第14届国际血小板免疫学研究会做为参比试剂,34个国际实验室基因分型鉴定结果的一致率为97.85%。采用EB病毒转化技术,能成功地血小板特异性抗原外周血淋巴细胞转化成永生化细胞淋巴母细胞株,细胞株稳定传代,并可用作为参比试剂。 |
| 关 键 词： | 血小板特异性抗原 EB病毒 永生化淋巴母细胞 参比试剂 |
Study on the stability of cell lines delivered from human paletlet-specific alloantigen and their apptications |
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| SHEN Wei-dong,LI Hai-yan,LI Li-lan,LIU Xue-jun,WU Guo-guang. Study on the stability of cell lines delivered from human paletlet-specific alloantigen and their apptications[J]. Current Immunology, 2010, 0(1) | |
| Authors: | SHEN Wei-dong LI Hai-yan LI Li-lan LIU Xue-jun WU Guo-guang |
| Affiliation: | SHEN Wei-dong,LI Hai-yan,LI Li-lan,LIU Xue-jun,WU Guo-guang(Nanning Institute of Transfusion Medicine,Nanning Blood Center,Nanning 530003,China) |
| Abstract: | To establish an immortal lymphoblastiod cell line of human platelet-specific alloantigen(HPA) and to use this cell line to provide perpetual research materials for long term studies,the immortal lymphoblastoid cell lines of human platelet specific alloantigen were established by Epstein-Barr virus(EBV) transformation of peripheral blood lymphocytes,in which 13 immortal lymphoblastoid cell lines of human platelet specific alloantigen were obtained successfully in the present study.All of the immortal lymphob... |
| Keywords: | human platelet specific alloantigen Epstein-Barr virus immortal lymphoblastoid cell lines reference materials |
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