红藤对小鼠的免疫抑制效应

目的：探讨红藤对小鼠免疫功能的影响。方法：以红藤水煎剂（25mg／100m1）给小鼠（18～22g）连续灌胃7d后，做碳廓清实验、抗体生成细胞的检测、淋巴细胞a-醋酸萘脂酶的检测、脾淋巴细胞转化实验以及迟发型变态反应（DTH）。结果：与对照组相比，红藤水煎剂能明显抑制小鼠单核一巨噬细胞的吞噬活性，降低小鼠机体抗体生成细胞分泌抗体IgM量，抑制小鼠外周血中的T淋巴细胞数目，降低脾淋巴细胞的转化率，减少DNCB致敏的小鼠两耳的重量差。经统计学处理，各实验组与对照组相比，各项指标均有极显著差异，P〈0．01。结论：红藤对小鼠细胞免疫功能及体液免疫功能均有明显抑郁作用，为今后中药免疫抑制剂的开发提供了科学的依据。

Immunosuppressive Effect of Caulis Sargentodoxae on Mice

Objective: To probe the immunosuppressive effect of caulis sargentodoxae on mice. Methods: Consistent gastric perfusion of canlis sargentodoxae 25 rag/100 ml were givern to mice( 18 ～ 22 g) for 7days to detect the earban clearance, antibodyproducing cells, lymphocyte a-naphtyl acetate esterase, transformation of spleen lymphocyte, delayed-type hypersensitivity. Results :Compared with control group, canlis sargentodoxae decoction inhibited the activity of mononuclear-macrophage, and decreased the antibody-secreted IgM content of antibody producing cells, inhibited the number of peripheral blood T lymphocyte, decreased the trasformation rate of spleen lymphocyte, decreased the weight difference of DNCB-sensitized mice ears. Compared with those of control group, all indexes of experiment groups had significantly statistic difference(P＜0.01). Conclusion: Caulis sargentodoxae has significant inhibiting effects on the immanofunction of cells and body fluids, which provides a sci-entific basis for the development of Chinese medicinal immunosuppressant.