大鼠脑缺血再灌注损伤IL-8变化的研究

目的：探讨白细胞介素0－8（interleukin-8,IL-8)在脑缺血再灌注损伤中的变化。方法：采用改良Zea Longa线栓法大鼠大脑中动脉闭塞（middle cerebral artery occlusion,MCAO)模型，将大脑中动脉（MCA）先行闭塞2小时，然后进行再灌注不同的时间，应用双抗体夹心间接ELISA法检测实验组和对照组大鼠脑组织及血清中IL－8浓度，结果：缺血2小时再灌注1小时脑组织IL－8含量较低（P＜0．05），随再灌注时间延长逐渐升高，至22小时达高峰，随后又降低；血清中IL－8含量于再灌注1小时是高，随后缓慢下降，再灌注46小时降至对照组水平；空白组，假手术组脑组织内IL－8含量高于缺血再灌注组（P＜0．01），结论：IL－8具有双重作用，既参与脑组织的正常代谢及生理功能，又参与了脑缺血再灌注损伤的病理生理过程。

The Change of the Content of Interleukin - 8 after Cerebral Ischemia - Reperfusion Injury in Rats

Objective To study the changes of the content of interleukin-8 (IL-8) in cerebrum and blood after cerebral ischemia-reperfusion injury. Methods The middle cerebral artery (MCA) was occlude with modified Zea Longa's rat MCAO/R model. The MCA was occluded for 2 hours and reperfused for different periods. The conternt of IL-8 in brain tissue and blood serum was detected by double antibody sandwich enzyme-linked immunosorbent assay. Results The content of IL-8 in brain tissue was lowered at 1 hour after reperfusion ( P <0.05). It increased with reperfusion, peaked at 22 hours, and then went down. The concentration of IL-8 in serum peaked at 1 hour after reperfusion, and then decreased. It reached the level of the control group at 46 hours after reperfusion. The level of IL-8 in brain tissue in control group was higher than that in experimental group ( P <0.01). Conclusion IL-8 might possess double roles: involved in metabolism and function of normal brain tissue and involved in the pathophysiological course of cerebral ischemia-reperfusion injury as well.