应用特异性单克隆抗体测定脑囊虫病患者血清及脑脊液中循环抗原

应用特异性单克隆抗体作DOT－ELISA和竞争ELISA检测65例脑囊虫病患者血清和脑脊液中游离循环Ag（FCAg）和循环免疫复合物抗原（CICAg）。血清FCAg阳性率分别为81．3％和81．5％（0．20～87．0μg／ml），CICAg阳性率为95.4％和92．3％（0.19～120.0μg／ml），脑脊液测定FCAg阳性单为78．5％和86.2％（0．32～25.0μg／ml），CICAg阳性率为90.8％和93.8％（0.19～46.4μg／ml）。结果表明检测总循环抗原（包括游离部分和复合物部分）有利于提高脑囊虫病的诊断。

Detection of Circulating Antigen of Neurocysticercosis Patients'Sera and Cerebrospinal Fluid with Monoclonal Antibody

Free and immune complex circulating antigens(FCAg and CICAg) sera and cerebrospinal fluid(CSF) from 65 neurocysticercosis patients were detected by DOT-ELISA and competitve ELISA using monoclonal antibodies. The positive rates (level) of FCAg were 81.3% and 81.5% (0. 2～87.0μg/ml) in sera and 78.5% and 86.2% (0.32～25. 0μg/ml) in CSF respectively;The positive rates(level) of CICAg were 95.4 % and 92.3% (0.19～128.0μg/ml) in sera and 90. 8% and 93. 8% (0. 19～46.4μg/ml) in CSF respectively. These results suggest that detection of total circulation antigen(FCAg and CICAg) from sera and CSF could contribute to diagnosis of neurocysticercosis.