利福平保护MPTP所致PD小鼠中脑多巴胺能神经元凋亡的实验研究

目的观察利福平(RFP)对1-甲基-4-苯基-1,2,3,6-四氢吡啶(MPTP)致帕金森病(PD) C57BL小鼠模型的神经保护作用。方法用MPTP建立C57BL小鼠PD模型,在应用MPTP前或后给予RFP,通过行为学观察、Nissl染色、免疫组织化学染色,观察RFP对PD小鼠模型的行为学表现及黑质致密部(SNc)Nissl、TH、Bcl-2、caspase-3阳性细胞的影响。结果小鼠注射MPTP后出现震颤、竖尾、竖毛、运动迟缓、步态不稳、肢体僵硬,活动明显减少,同时SNc的Nissl、TH阳性细胞明显减少,Bcl-2阳性细胞有所增多,caspase-3阳性细胞明显增多；应用RFP后较MPTP组症状减轻,Nissl、TH、Bcl-2阳性细胞增多．而caspase-3阳性细胞减少；预使用RFP组作用最为明显。结论RFP对MPTP所致的PD小鼠中脑多巴胺能神经元凋亡具有保护作用。

Neuroprotective effects of Rifampicin on MPTP-induced DA neurons apoptosis in C57BL mice

Objective To study the neuroprotective effects of Rifampicin(RFP)on l-methyl-4-phenyl-1,2,3,6-tetrahydropyridine(MPTP)-induced Parkinson's disease(PD)in C57BL mice. Methods The PD models were formed with intraperitoneal injections of MPTP.RFP was administered intraperitoneally to mice prior or posterior to MPTP.The effects of MPTP on the behavioral exhibition were explored.The number of dopamine(DA)neurons in the compact part of the substantia nigra(SNc)were studied by Nissl and immunohistochemical analysis.Results After MPTP injection,the mice behaved with tremor,erect tail,piloerection,bradykinesia,instability of gait and hypokinesia.The number of both Nissl and TH positive cells decreased obviously.However the number of Bcl-2 positive cells and caspase-3 positive ones increased obviously in the SNc.RFP given posterior to MPTP relieved the symptom,the number of Nissl,TH,Bcl-2 positive cells increased and the caspase-3 positive cells decreased in the SNc. RFP given prior to MPTP had the most effect.Conclusion RFP is neuroprective against MPTP-induced DA neurons apoptosis in C57BL mice.