血浆中盐酸噻氯吡啶的高效液相色谱法及其在相对生物利用度研究中的应用

 目的：建立测定血浆中的盐酸噻氯吡啶浓度的反相高效液相色谱法。方法：采用C₁₈柱，流动相为甲醇-水-浓氨水（780∶220∶9）。200μl血浆用乙腈沉淀蛋白后，上清液直接进样。结果：本方法在0．1mg·L^－1～2．0mg·L^－1范围内线性良好，最低检测浓度为0．06mg·L^－1。批间和批内误差均小于10％。方法回收率平均值为100．18％。结论：此方法具有操作简便、省时、分离度较好等特点。已经应用于正常人多剂量口服盐酸噻氯吡啶片剂后的相对生物利用度和药动学研究。

The HPLC assay of ticlopidine in plasma and its application in relative bioavailability study

OBJECTIVE: To determine the ticlopidine concentration in human plasma. METHOD: Reverse-phase high-performance liquid chromatographic assay with C₁₈ column was developed. 200 μl plasma was extracted with acetonitrile followed by injecting the supernatant directly. RESULTS: The linearity ranged from 0.1 to 2.0 mg·L^-1. The detection limit was 0.06 mg·L^-1. The RSD of intra-run and inter-run was less than 10%. CONCLUCIONS: This assay is rapid, accurate, sensitive, and simple. It has been used in evaluating the relative bioavailability of ticlopidine tablet and its pharmacokinetics.