Subcellular Phototoxicity of Photofrin-II and Lutetium Texaphyrin in Cells In Vitro |
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Authors: | H Liang DS Shin YE Lee DC Nguyen S Kasravi T Do P Aurasteh MW Berns |
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Institution: | (1) Beckman Laser Institute and Medical Clinic, University of California, Irvine, California, USA, US |
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Abstract: | . Three cell types including bovine pulmonary artery endothelium cells (CPAE), rat kangaroo kidney cells (PTK2), and human larynx epidermoid carcinoma cells (Hep-2) were used to study subcellular localisation and phototoxicity of Photofrin-II
and lutetium texaphyrin (Lu Tex). Cells were examined for fluorescence after administration of the photosensitisers. Subcellular
regions were exposed with a laser microbeam system that used an argon ion laser pumped dye laser generating a 630 nm for Photofrin-II
and 730 nm for Lu Tex. Fluorescence detection suggests that the Photofrin-II is bound primarily to the mitochondria with some
diffuse fluorescence in the rest of the cytoplasm. The fluorescence in Lu Tex treated cells appears to be localised to the
lysosomes. The percentage of damaged cells following light exposure to the different subcellular regions after Photofrin-II
or Lu Tex treatment demonstrates that the nuclear region was the most sensitive target followed by the perinuclear region
and peripheral cytoplasm region.
Paper received 27 January 1998; accepted after revision 21 August 1998. |
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Keywords: | : Endothelial cell Fluorescence detection Laser microirradiation Lutetium texaphryin Photodynamic therapy Photofrin II Photosensitiser Subcellular phototoxicity |
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