人脂肪组织来源干细胞植入裸鼠的成脂效应

目的 探讨脂肪抽吸术液态部分获取的人脂肪组织来源干细胞(adipose tissue-derivedstem cells,ADSC)植入裸鼠的成脂效应.方法 自人体脂肪抽吸术中液体部分分离、培养和鉴定ADSC,行成脂、骨和软骨分化.植入物分三组:空支架(第1组),人ADSC接种胶原海绵(第2组),经成脂诱导的ADSC接种胶原海绵架(第3组),植入裸鼠皮下.在2和8周时,通过组织观察、HE染色和油红O染色来分析新生组织.结果 抽脂术液态部分能获取大量的ADSC,能分化成脂肪、成骨和软骨细胞.8周时,第1组植入物已降解,第2组和第3组均有脂肪组织形成,但第2组比第3组在形成体积上小、成脂效应差.结论 从脂肪抽吸术液态部分分离和培养的ADSC和胶原支架混合培养能在体内形成脂肪组织,此方法 获得的ADSC多能干细胞可作为脂肪组织工程的种子细胞.

Adipogenic effect of human adipose tissue derived stem cells in nude mouse

Objective To research adipogenie effect of human adipose tissue derived stem cells(ADSC)obtained from fluid portion of liposuetion aspirates in nude mouse.Methods ADSC were isolated from the fluid portion of human liposuetion aspirates and cultured.The cells were incubated in either a adipogenie or an osteogenie medium or a ehondrogenic medium for 2-4 weeks to induce adipogenesis or osteogenesis or ehondrogenesis,respectively.Adipogenesis WaS examined in nude mice imbeded subcutaneously with collagen sponge(group 1),or collagen sponge seeded with ADSC(group 2),or collagen sponge seeded with adipogenic differentiated ADSC(group 3).Histologic examination,HE and oil red 0 staining were used to assess the newly formed tissue.Results A large number of ADSC were obmined from the fluid portion of human hposuetion aspirates.The ADSC were differentiated into adipose tissue,bone and cartilage.8 weeks after implantation,the collagen sponge WaS folly absorbed in group 1.Newly formed adipose tissue was observed in groups 2 and 3,but much more in group 3.Conclusions The ADSC from the fluid portion of human liposuction aspirates can be cultured with collagen framework to form adipose tissue in vivo.It indicates that ADSC can be used as seed cells in adipose tissue engineering.