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| KF-950对人精子顶体素活性及顶体的影响 | |
| 引用本文： | 詹瑛,赵亚南,李宗林,李丽,宋谦.KF-950对人精子顶体素活性及顶体的影响[J].中华男科学杂志,2002,8(2):92-94. |
| 作者姓名： | 詹瑛 赵亚南 李宗林 李丽 宋谦 |
| 作者单位： | 1. 解放军401医院妇产科,山东,青岛,266071 2. 上海长海医院全军生殖医学研究中心,上海,200433 3. 复旦大学生命科学院遗传研究所,上海,200433 |
| 基金项目： | 上海市自然科学基金课题 (93 1960 790 ) |
| 摘 要： | 目的 :研究顶体素抑制剂 4 胍基苯甲酸盐 (KF 95 0 )对人精子顶体素的抑制作用和对顶体的影响。 方法 :醋酸抽提并纯化人精子顶体素 ,BAEE/ADH法测定不同剂量KF 95 0作用后残余酶的活性。以生物素标记的豌豆凝集素为探针 ,采用ABC染色法观察KF 95 0对完整精子顶体的影响。 结果 :①正常人精子顶体素活性为(37.6 5± 4 .4 7)U/L(pH 8.5 ,2 5℃ ) ;②残余酶活性与KF 95 0剂量呈负相关 (r =- 0 .998) ,并呈量 效依赖关系 ,回归方程 :Y =7.5 7- 1.895X ;③随药物浓度的增加和作用时间的延长 ,顶体完整着色率均明显下降 (P <0 .0 1)。结论 :KF 95 0能直接抑制顶体素活性 ,推测对精子顶体也有明显的破坏作用 ,是一种新型、高效的精子顶体素抑制剂。 |
| 关 键 词： | 顶体素抑制剂 4胍基苯甲酸盐 顶体素 顶体 精子 |
| 文章编号： | 1009-3591(2002)02-0092-03 |
| 修稿时间： | 2001年8月27日 |
Effect of Acrosin Inhibitor KF-950 on Acrosin Activity and Acrosome of Human Sperm |
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| Ying ZHAN ,Ya Nan ZHAO ,Zong Lin LI ,Li LI ,Qian SONG.Effect of Acrosin Inhibitor KF-950 on Acrosin Activity and Acrosome of Human Sperm[J].National Journal of Andrology,2002,8(2):92-94. | |
| Authors: | Ying ZHAN Ya Nan ZHAO Zong Lin LI Li LI Qian SONG |
| Institution: | Department of Obstetric and Gynecology, 401st Hospital of PLA, Qingdao, Shandong 266071, China. |
| Abstract: | OBJECTIVES: To study the inhibitory effect of KF-950 on human acrosin and sperm acrosome. METHODS: Human acrosin was extracted and purified with 2% acetic acid, and its residual activity was evaluated by BAEE/ADH assay after treated with different concentrations of KF-950. ABC assay was used to observe the effect of KF-950 on human acrosome with Biotin-PSA as a probe. RESULTS: 1. The activity of normal sperm acrosin was (37.65 +/- 4.47) U/L. 2. The residual activity was inversely related to the concentration of KF-950 (r = -0.998), and had a dose-response curve. The result could be described by Y = 7.57-1.895X. 3. With increase of KF-950 concentration and prolongation of action time, the staining rate of acrosome obviously dropped (P < 0.01). CONCLUSIONS: KF-950 directly inhibits acrosin activity and assumely injures sperm acrosome. It might be a new kind of highly effective inhibitor. |
| Keywords: | Acrosin inhibitor 4 Guanidinobenzoat Acrosin Acrosome Spermatozoa |
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