电离-飞行时间-质谱技术分析免疫大鼠血清和脑脊液的变化

目的研究神经免疫调节网络的上传信号及信号传导途径。方法应用表面加强激光解析电离-飞行时间-质谱技术(SELDI-TOF-MS)对不同免疫状态大鼠的外周血和脑脊液组分进行比对分析,确定信号分子。结果在免疫大鼠外周血清中发现了221个具统计学意义的差异峰,其中184个为正向表达峰值,37个峰为对照组表达增高的负向峰值。实验各组大鼠脑脊液中未见有统计学意义的特异性差异峰表达,在血清中检测到的特异性差异峰在脑脊液中也未见相对应的同步增高。结论在神经免疫调节网络中,由免疫调质直接透过血-脑脊液屏障构成网络传入通路的证据不足。

The Diversity of Immunized Rats Serum and Cerebrospinal Fluid Analyzed by SELDI-TOF-MS

Objective To study the up-delivering signal in neuroimmunoregu-lation network and signal transduction pathway involved.Methods Contrast analysis was adopted in the ingredients of peripheral blood and cerebrospinal fluid of different immunized stage rats by surface enhanced laser desorption ionization-time of flight-mass spectrometry(SELDI-TOF-MS)technique to determine signal molecules.Results A total of 221 significant different peaks were discovered in peripheral serum of immune animals by SELDI.Among the total of 221 different peaks,184 peaks were high expressions in experimental groups and the others were reverse-increases in control groups.But no special different peaks which had statistical significance were found in cerebrospinal fluid of immune animals.Also corresponding equal increase couldn't be observed between the special different peaks tested in peripheral serum and cerebrospinal fluid of immune animals.Conclusions These foregoing results demonstrated that there was lack of evidence to verify immune modulator could carry out neuro-immune modulation by cerebrospinal fluid passing through blood brain barrier directly.