高效液相法测定陈皮药渣中橙皮苷含量

摘 要 目的:通过对陈皮药材及药渣中橙皮苷含量进行比较分析，为陈皮药渣的二次开发提供依据。方法: 色谱柱:Eclipse XDB- C₁₈(280 mm×4.6 mm, 5 μm)流动相：甲醇∶水∶冰醋酸（30∶66∶4）；流速：1.0 ml·min^-1；检测波长：283 nm。结果:橙皮苷与其它色谱峰分离良好。橙皮苷的线性范围在0.42～4.20 μg,r=0.999 7。陈皮药材的平均加样回收率为98.5%，RSD=2.12%(n=6);陈皮药渣的平均加样回收率为98.1%，RSD=2.35%(n=6)。结论:陈皮药渣中橙皮苷的提取量为陈皮药材中橙皮苷的提取量52%，表明陈皮药渣具有二次开发利用的价值。

Determination of Hesperidin in Dregs of Pericarpium Citri Reticulatae by HPLC

Objective： To compare and analyze the content of hesperidin in Pericarpium Citri Reticulatae and its residues to provide the basis for the secondary development of Pericarpium Cirri Reticulatae residues. Method： Eclipse XDB-C18 （ 250 mm × 4.6 mm, 5 μm）column was used. The mobile phase was methanol-water-acetic acid （30： 66： 4）. The detection wavelength was 283 nm and the flow rate was 1.0 ml · min ^- 1. Result： The linear range was 0.42 - 4.20 μg （ r = 0. 999 7 ）. The average recovery of Pericarpium Citri Reticulatae was 98.5 % with RSD of 2.12% （ n = 6）. The recovery of the dregs was 98.1% with RSD of 2.35 % （ n = 5）. Conclusion ： Hesperidin in the residues is 52% higher than that in Pericarpium Citri Reticulatae. The result shows that the residues have great secondary development value.