HPLC测定鸡血藤片中原儿茶酸的含量

摘 要 目的:建立鸡血藤片中原儿茶酸含量的高效液相色谱法。方法: 采用Inertsil C₁₈（250 mm×4.6 mm 5 μm）色谱柱，以甲醇∶水∶冰醋酸（25∶75∶0.2）为流动相，检测波长为260 nm，流速为0.8ml·min^-1。结果:原儿茶酸进样量在0.010～0.063 μg范围内与峰面积线性关系良好，r=0.999 4（n=6）。方法平均回收率为99.23%，RSD为0.35%。结论:高效液相色谱法简便、灵敏、稳定、准确，无其他成分干扰，可作为鸡血藤片质量控制的有效方法。

Determination of Protocatechuic Acid in Jixueteng Tablets by HPLC

Objective： To establish an HPLC method for determining the content of protocateehuic acid in Jixueteng tablets. Meth- od： The HPLC method was adopted and the Inertsil Cls column（250 mm ×4.6 mm,5 μm）was used. The mobile phase was methyl alcohol-water-aceticacid（25： 75： 0.2）. The detection wavelength was at 260 nm and the flow rate was 0.8 ml ·min^-1. Result： The linear range of protocatechuic acid was 0. 010-0.063 μg, r = 0.999 4. The average recovery was 99.23 % with RSD of 0.35 % （ n = 6）. Conclusion： The method is simple, sensitive, stable and accurate, which can be used for the quality control of Jixueteng tablets.