HPLC法测定五味清浊散中羟基红花黄色素A的含量

摘 要 目的:建立五味清浊散中羟基红花黄色素A的高效液相色谱测定方法。方法: 色谱柱为Prevail Select C₁₈（150 mm ×4.6 mm，5 μm），柱温35℃；流动相为乙腈-0.5% 磷酸溶液 (11∶89)，流速 1 ml·min^-1；检测波长403 nm。结果:羟基红花黄色素A在16.65～166.50 μg·ml^-1范围内呈良好的线性关系，回归方程为Y=3.24×10^-2X+2.12,r=0.999 9。平均回收率为99.1%，RSD为1.9%。结论:本方法操作简便，测定结果准确可靠，可用于五味清浊散中羟基红花黄色素A的含量测定。

Determination of Hydroxysafflor Yellow A in Wuwei Qingzhuo Powders by HPLC

Objective： To establish an HPLC quantitative method for the determination of hydroxysafflor yellow A in Wuwei Qingzhuo powders. Method： High Performance Liquid Chromatography was used for the determination of hydroxysafflor yellow A. The chromatography column was Prevail Select C18 （150 mm × 4.6 mm,5 μm）and the column temperature was 35℃. The mobile phase was acetonitrile-0.5 % phosphoric acid （ 11： 89）. The flow rate was 1 ml ·min^-1 and the detection wavelength was at 403 nm. Result： The retention time of hydroxysafflor yellow A was about 14 min with resolution above 1.5. The regress equation for hydroxysafflor yellow A was as the following ： Y = 3.24 × 10^ -2X ＋ 2.12 （ r = 0.999 9）, and the linear range was 16.65-166.50 μg · ml ^- 1. The average recovery of hydroxysafflor yellow A was 99.1% （ RSD = 1.9% ）. Conclusion ： This method is simple, time-saving and accurate, It can be used for the routine analysis of hydroxysafflor yellow A in Wuwei Qingzhuo powders.