| 注射狂犬疫苗饮酒引起过敏反应一例报告 |
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| 引用本文： | 宫仁梅,万超群.注射狂犬疫苗饮酒引起过敏反应一例报告[J].疾病监测,1996,11(2):68-67. |
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| 作者姓名： | 宫仁梅 万超群 |
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| 作者单位： | 1.黑龙江穆棱市卫生防疫站（157500）;八面通林业局防疫站 |
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| 摘 要： | 本文建立了一个用于检测军团菌的多聚酶链反应方法。扩增参考菌株的染色体DNA(L.pneumophital-14、L.micdadei、L.dumoffii Llongbeachae、L.Jordanis、L.bozemanii)，均可检出375bp的16SrRNA基因片段。对于已经监定的5株国内分离菌株染色体DNA进行扩增，获得了相同的结果，地高辛标记16SrRNA基因探针与扩增产物杂交，结果为阳性。而扩增14株非军团菌均为阴性。采用煮沸法制备细菌染色体DNA,PCR法检测环境水军团菌敏感性为280cfu/ml水，检查临床标本军团菌为560cfu/ml支气管灌洗液。上述结果表明该法敏感、快速、简便，具有较好的特异性。
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| 关 键 词： | 16SrRNA 基因 军团菌 聚合酶链反应 |
| 收稿时间： | 1995-12-01 |
Establishment and Preliminary Application of a 16SrRNA Polymerase Chain Reaction Assay for Detection of Legionella Species |
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| Gong Shimei et al.Establishment and Preliminary Application of a 16SrRNA Polymerase Chain Reaction Assay for Detection of Legionella Species[J].Disease Surveillance,1996,11(2):68-67. |
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| Authors: | Gong Shimei |
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| Institution: | 1.Institute of Epid emiology and Microbiology;Chinese Acad emy of Preventive Medicinec;Beijing 102206 |
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| Abstract: | A 165rRNA polymerasse chain reaction CPCR) assay for the detection of Le- gionella Species was established. A 375 bp fragment of the 16SrRNA gene could be detected by amplifing genomic DNA of reference strains (Lp1-14, Lm, L1, Ld, Lb, Lg). The same result was also obtained by amplifing 5 identified strains isolated from China. The amplified products were verified by hybridization with 16SrRNA gene probes. However, 14 non-legionella strains could't produced a positive amplified fragment. Preparing bacterial genomic DNA by boiling method,the assay could detect 280cfu/ml water and 560 cfu/ml clinical bronchial fluid respectively. Above- mentioned result showed that this assay was sensitive、rapid、specific and easy to perform. |
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| Keywords: | SrRNA gene Legionella Polymerase chain reaction (PCR) |
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