金黄地鼠颊囊黏膜癌变过程中iNOS的表达与Bcl-2的关系

目的：观察金黄地鼠颊囊黏膜癌变过程中诱导性一氧化氮（inducible nitric oxide synthase，iNOS）的表达与凋亡相关蛋白Bcl-2的关系。方法：6—8周龄金黄地鼠72只，随机分为实验组（60只）和空白对照组（12只），空白对照组地鼠不做任何处理．实验组地鼠用0．5％的二甲基苯丙蒽（dimethyl-benzanthrance，DMBA）丙酮溶液诱导生成颊囊黏膜癌．并在黏膜癌变的不同阶段检测组织中iNOS和Bcl-2的表达。结果：正常地鼠颊囊黏膜组织中未见iNOS阳性表达．组织中iNOS、Bcl-2的表达强度在颊囊黏膜癌变过程中均呈上升趋势。鳞癌组织中iNOS的表达较单纯增生组织显著增强（P〈0．011；鳞癌组织中Bcl-2的表达强度明显高于轻度上皮异常增生组（P〈0．05），但与重度和中度上皮异常增生组之间无显著性差异（P〉0．05）；Bcl-2的表达强度随iNOS表达强度的增强呈现先升后降的复杂变化。结论：一氧化氮参与了颊囊黏膜鳞癌的发生、发展，并可能通过Bcl-2途径调节肿瘤细胞的凋亡。

Correlation Between Nitric Oxide Synthase and Bcl-2 Expressions in Hamster Buccal Pouch Carcinogenesis

Objective:To observe the correlation of inducible nitric oxide synthase(iNOS)with Bcl-2 in the golden hamster buccal pouch carcinogenesis induced hy dimcthyl-benzanthrance(DMBA).Methods:72 outbred six-week old Syrian gohten hamsters were randomly divided into experimental group(60 animals)and control group(12 animals).The pouches of the experimental hamsters were painted with 0.5% DMBA solution three times a week.Light microscope was used to observe the pathologic changes.Expressions of iNOS and Bel-2 were observed hy immunohistochemistry.Results: In oral carcinoma group iNOS and Bcl-2 protein expression were significantly increased compared with the normal or hyperplasia group(P<0.05).There was complicated relationship between nitric oxide and Bel-2.Conclusion:Nitric oxide might suppress or induce cell apoptosis by regulating apoptotic signaling cascades in Bcl-2.