| Allo-PBSCT患者CD4＋CD25＋调节性T细胞的体外研究 |
| |
| 引用本文： | 翟海龙,赖永榕.Allo-PBSCT患者CD4＋CD25＋调节性T细胞的体外研究[J].山东医药,2011,51(33):10-12. |
| |
| 作者姓名： | 翟海龙 赖永榕 |
| |
| 作者单位： | 1. 黄石理工学院医学院,湖北黄石,435003
2. 广西医科大学第一附属医院 |
| |
| 摘 要： | 目的探讨异基因外周血干细胞移植（Allo-PBSCT）患者外周血CD4＋CD25＋调节性T细胞（Tregs）在协同刺激信号作用下的增殖反应、与CD4＋CD25-T细胞混合淋巴细胞反应及上述两种培养细胞的细胞因子分泌情况。方法对36例Allo-PBSCT患者离体CD4＋CD25＋Tregs在抗CD3-mAbs和抗CD28-mAbs的刺激下行CD4＋CD25＋Tregs培养和CD4＋CD25＋Tregs、CD4＋CD25-T细胞混合淋巴细胞培养72 h,然后加入CCK-8溶液孵育1 h,用酶标仪检测OD450值,同时测量上述两种细胞培养上清液中IL-10、TGF-β和IFN-γ的浓度。另以7例健康者为对照组。结果 CD4＋CD25＋Tregs细胞OD450值低于CD4＋CD25-T细胞（P〈0.01）。CD4＋CD25＋Tregs与CD4＋CD25-T细胞混合OD450值也低于CD4＋CD25-T细胞（P〈0.01）。有移植物抗宿主病（GVHD）和无GVHD患者离体CD4＋CD25＋Tregs在抗CD3-mAbs和抗CD28-mAbs的共刺激下分泌的IL-10、TGF-β和IFN-γ低于CD4＋CD25-T细胞（P〈0.01）,有GVHD和无GVHD患者的两细胞混合组分泌的这3种细胞因子均低于CD4＋CD25-T细胞（P〈0.05）,Allo-PBSCT患者分泌细胞因子情况与对照组类似。结论有效地利用Allo-PBSCT受者外周血CD4＋CD25＋Tregs的抑制功能,则有可能降低Allo-PBSCT后GVHD的发病率。
|
| 关 键 词： | 干细胞移植 异基因 移植物抗宿主病 CD4＋CD25＋调节性T细胞 增殖 体外 |
Study on post-allogeneic peripheral blood stem cell transplantation patients' CD4~+CD25~+ regulatory T cells in vitro |
| |
| ZHAI Hai-long,LAI Yong-rong.Study on post-allogeneic peripheral blood stem cell transplantation patients' CD4~+CD25~+ regulatory T cells in vitro[J].Shandong Medical Journal,2011,51(33):10-12. |
| |
| Authors: | ZHAI Hai-long LAI Yong-rong |
| |
| Institution: | ZHAI Hai-long1,LAI Yong-rong(1 Medical College of Huangshi Institute of Technology,Huangshi 435003,P.R.China) |
| |
| Abstract: | Objective To investigate the proliferation reaction of CD4+CD25+ Tregs in the stimulating of costimulatory signal,lymphocyte reactions mixed with CD4+CD25-T cells of CD4+CD25+ Tregs,and cytokine secretion state of the two cells in allogeneic peripheral blood stem cell transplantation(Allo-PBSCT) patients.Methods CD4+CD25+Tregs and CD4+CD25-T cells from peripheral blood obtained from 36 patients who had undergone Allogeneic peripheral blood stem cell transplantation(Allo-PBSCT),7 healthy volunteers as contro... |
| |
| Keywords: | stem cell transplantation allogeneic GVHD CD4 CD25 regulatory T cells proliferation in vitro |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
