吸烟对氧自由基及抗氧化酶活性影响的研究

目的　探讨吸烟与氧自由基（OFR）及抗氧化酶活性的关系。方法　对216例健康吸烟者血清过氧化脂质(LPO) 含量、超氧化物歧化酶(SOD) 、谷胱苷肽过氧化物酶（GSH-PX）活性进行检测，并进行对比分析。结果　吸烟组LPO为 (5.87±1.23umol/L) ，SOD为(88.24±11.96uU/L)，GSH-PX为(176.87±23.31U)；非吸烟组LPO为 (4.24±0.76umol/L) ，SOD为(106.18±16.04uU/L) ，GSH-PX为 (206.32±50.25U)。两组比较吸烟组LPO含量显著升高(P<0.01)，而SOD、GSH-PX活性显著降低(P<0.01)， 其中50例36～50岁男性吸烟者随其吸烟年限和吸烟量的增加，均呈相关性(P<0.01)。结论　吸烟可引起人体内OFR和LPO反应加剧，是导致氧化和抗氧化平衡失调的主要原因之一。

Effects of cigarette smoking on oxygen free radicals andactivities of antioxidases

Objective　To study the relationship between smoking and oxygen free radicals (OFR) , activities of antioxidases.Methods Concentration of serum lipoperoxides(LPO) and activity of superoxide dismutase(SOD) ,glutathioneperoxides(GSH-PX) in 216 healthy smoker and 155 healthy non-smoker were performed.Results The smoker group: the averageLPO concentration was 5.87±1.23, the average SOD activity was 88.24±11.96, the average GSH-PX activity was 176.87±23.31;the non-smoker group:The average LPO concentration was 4.24±0.76, the average SOD activity was 106.18±16.04, the average GSH-PX activity was 206.32±50.25.The average LPO concentration of the smoker group was significantly higher than that in the non-smoker group (P<0.01) ; the average SOD GSH-PX activity of the smoker group were significantly lower than those of the non-smoker group (P<0.01) .The LPO concentration in 50 male smokers(39～45years of age)increased, while SOD and GSH-PX activity decreased as the duration and amount of smoking increased (P<0.01) , showing a linear correlationship.Conclusion The results suggest that smoking is one of the important factors causing, not only OFR and reaction of LPO obviously intensified,but also the imbalance between oxidation and antioxidation in the body.