套管法建立同系与异系大鼠颈部心移植模型的比较

目的:观察套管法建立同系与异系大鼠颈部心移植模型后的动物存活与形态学变化,进而探讨该方法的操作技巧,以提高成功率.方法:套管法制备同系(Wistar-Wistar)与异系(Lewis-Wistar)大鼠颈部心移植模型,另对异系移植动物行腹腔注射环胞霉素.术后观察移植物的搏动,并记录每组动物存活时间.移植后7d,移植心切片行H-E染色,按照国际心脏与肺移植协会制定的标准对其组织病理变化进行分级.结果:同系、异系动物间移植后,其存活率分别为(27.5±6.1)d和(7.2±1.3)d.环胞霉素的施加,可将异系移植的存活时间提高至30d以上.异系移植可致明显急性排斥反应,其病理分级以4级为主,远较同系移植和环胞霉素处理为高.结论:环胞霉素可有效缓解因异系心移植所致的急性排斥反应,延长动物存活时间.在确保良好供心的基础上,防止出血和血栓形成是模型成功制备的关键.

Comparison on rat cervical hearts heterotopically transplantation by cuff technique

Objective: To investigate the survival and histopathological changes of heterotopic heart transplanted using cuff technique, therefore to discuss the operative skills in order to improve the success rate. Methods: Using the cuff technique, the cervical heterotopic heart transplantation was performed from Wistar to Wistar rats (isograft), Lewis to Wistar rats (allograft) treated with or without intraperitoneal injection of cyclosporine A. The heart beat was observed and survival time of experimental animals was recorded. The sections of transplanted hearts were postoperatively performed at day 7, and subsequently stained using HE staining to observe microstructures of myocardium, thereafter, were analyzed according to the ISHLT grading system. Results: Survival time of isografted and allografted rats was (27.5±6.1) and (7.2±1.3) days, respectively. Intraperitoneal injection of gyclosporine A caused prolonged survival time (>30d) of allografted rats.Allograft lead to a severely acute immunological rejection, the ISHLT scores of allografted rats were dominantly Grade 4, which was significant higher than those in isografted rats (1A-1B) and allografted rats treated with gyclosporine A (2-3B). Conclusion: Administration of cyclosporine A can relieve the acute immunological rejection of allografted rats, resulting in elongation of the survival time.Prevention of hemorrhage and thrombosis is the pivotal issue to made a successful model, on the premise that the ideal donor heart is provided.