白细胞滤器在狗失血性休克实验性治疗中的作用

目的　研究在犬失血性休克条件下白细胞对机体重要脏器的损伤以及白细胞滤器减轻损伤的作用。方法　建立犬失血性休克模型 ,将 12只雄性犬分为空白组、输注全血组、输注去白组 ,在失血后 2h分别回输等量生理盐水、全血、去白细胞全血 ,观察输血 (液 )后血生化指标及心、肝、肾、肺的病理变化。结果　白细胞计数、血清LDH、GOT、MDA水平全血组较去白组显著升高(P <0 0 5 ) ;形态学上 ,肝、肾损伤全血组较去白组严重。结论　输注去白细胞血液较输注全血能明显减轻激活白细胞对重要脏器的损伤作用。

Effect of Leukocyte Depletion Filter During the Treatment of Canine Hemorrhagic Shock

Objective To study the injurous effect of leukocytes on vital organs of hemorrhagic shock dog and role of leukocyte depletion filter in reducing the injury.Methods A canine model of hemorrhagic shock was established.12 male dogs were divided into blank group,whole blood transfusion group and leukocyte depletion group. Same amount of 0 9% physiological saline,whole blood of the canine itself and filtrated whole blood (i.e. lack of leukocytes) were back transfused respective correspondingly, changes in parameters such as white blood cells counting, LDH, MDA, GOT and the pathological changes in heart, lung, liver and kidney were detected among different treatment groups.Results Higher level of WBC,LDH, AST and MDA were observed in the group with whole blood transfused than in the group of leukocyte-depletion transfused. The pathological changes in liver and kidney indicated more serious injury happened in whole blood group than in the leukocyte-depletion group.Conclusion The Leukocyte depletion filter in itself can definitely alleviate the damages in the canine vital organs when reperfusion is inevitable as a result of hemorrhagic shock.